Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (undifferentiated human iPS cells)
Specification
undifferentiated human iPS cells
Blocking step
5% milk in TBST as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
1 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% milk in TBS
Secondary antibody
Name
Non-Abcam antibody was used: Dako Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 85, 140 kDa Non-specific: 180, 65 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Undifferentiated human iPS cells were lysed in RIPA buffer with protease inhibitors and performed under reducing conditions (10% gel). Following block overnight the samples were probed using an anti Sall4 antibody (ab29112) at 1ug/ml, followed by incubation with the relevant secondary antibody at 1:2000. Samples were visualised using ECL for 5 mins and specific bands at approx 140 and 85kDa were observed. However, some additional non specific bands were observed 180 and 65 kDa.
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提交于 Dec 18 2013