Anti-SAFB抗体(ab97520)
Key features and details
- Rabbit polyclonal to SAFB
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-SAFB抗体 -
描述
兔多克隆抗体to SAFB -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Cow
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免疫原
Recombinant protein fragment corresponding to a region within amino acids 476-705 of Human SAFB (NP_002958).
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阳性对照
- A431, H1299, HeLa and HepG2 cell lysates for WB; HeLa cells for ICC/IF
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97520于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 103 kDa.
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| ICC/IF |
1/100 - 1/200.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 103 kDa. |
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ICC/IF
1/100 - 1/200. |
靶标
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功能
Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing (By similarity). Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. -
组织特异性
Ubiquitous. Expressed at high levels in the CNS and at low levels in the liver. Expressed in a wide number of breast cancer cell lines. -
序列相似性
Contains 1 RRM (RNA recognition motif) domain.
Contains 1 SAP domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6294 Human
- Entrez Gene: 224903 Mouse
- Entrez Gene: 64196 Rat
- Omim: 602895 Human
- SwissProt: Q15424 Human
- SwissProt: Q80YR5 Mouse
- SwissProt: O88453 Rat
- Unigene: 728802 Human
see all -
别名
- DKFZp779C1727 antibody
- glutathione S transferase fusion protein antibody
- HAP antibody
see all
图片
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Western blot - Anti-SAFB antibody (ab97520)Anti-SAFB antibody (ab97520) at 1/1000 dilution + A431 whole cell lysate at 30 µg
Predicted band size: 103 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-SAFB antibody (ab97520)ab97520, at a 1/100 dilution, staining SAFB in paraformaldehyde fixed HeLa cells by Immunofluorescence analysis.
Lower image was merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab97520 尚未被引用在任何文献中。