Anti-SA2抗体(ab4464)

Submit a review Q&A (1)References (3)
Immunocytochemistry - Anti-SA2 antibody (ab4464)
  • Western blot - Anti-SA2 antibody (ab4464)

Key features and details

  • Goat polyclonal to SA2
  • Suitable for: WB, ICC
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-SA2抗体
    参阅全部 SA2 一抗

  • 描述

    山羊多克隆抗体to SA2

  • 宿主

    Goat

  • 经测试应用

    适用于: WB, ICCmore details

  • 种属反应性

    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan

  • 免疫原

    Synthetic peptide (Human) - 31 amino acids which represent a portion of human SA 2 encoded within exon 31.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab4464于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
ICC
1/500 - 1/2000.

靶标

  • 功能

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.

  • 序列相似性

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.

  • 翻译后修饰

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.

  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • bA517O1.1 antibody
    • Cohesin Subunit SA 2 antibody
    • Cohesin subunit SA-2 antibody
    • DKFZp686P168 antibody
    • DKFZp781H1753 antibody
    • FLJ25871 antibody
    • SA 2 antibody
    • SA-2 antibody
    • SA2 antibody
    • SCC3 homolog 2 antibody
    • SCC3B antibody
    • STAG 2 antibody
    • stag2 antibody
    • STAG2_HUMAN antibody
    • Stromal antigen 2 antibody
    see all

图片

  • Immunocytochemistry - Anti-SA2 antibody (ab4464)
    Immunocytochemistry - Anti-SA2 antibody (ab4464)
    Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4464 at 1µg/ml.
  • Western blot - Anti-SA2 antibody (ab4464)
    Western blot - Anti-SA2 antibody (ab4464)

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4464 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4464 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

文献 (3)

发表研究结果有使用 ab4464？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4464 被引用在 3 文献中.

  • Park SH  et al. ATAD5 promotes replication restart by regulating RAD51 and PCNA in response to replication stress. Nat Commun 10:5718 (2019). PubMed: 31844045
  • Faure AJ  et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
  • Inoue A  et al. Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation. Cell Cycle 6:1646-54 (2007). PubMed: 17611414

客户评价及客户问答

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Question

I have a question related to the four types of goat anti-SCC3 antibodies that you have (Ab4455, Ab4457, Ab4463, and Ab4464). Do you know which one works best on immunostaining?

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Answer

Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

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