Key features and details
- Goat polyclonal to SA2
- Suitable for: WB, ICC
- Reacts with: Human, Xenopus laevis
- Isotype: IgG
参阅全部 SA2 一抗
经测试应用适用于: WB, ICCmore details
种属反应性与反应: Human, Xenopus laevis
预测可用于: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide (Human) - 31 amino acids which represent a portion of human SA 2 encoded within exon 31.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab4464 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).|
|ICC||1/500 - 1/2000.|
功能Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
序列相似性Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain.
翻译后修饰Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
细胞定位Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex.
- Information by UniProt
- bA517O1.1 antibody
- Cohesin Subunit SA 2 antibody
- Cohesin subunit SA-2 antibody
Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA2 with ab4464 at 1µg/ml.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4464 at 0.5
Detection: ECL with 15 second exposure.Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4464 at 0.5 µg/ml.
Detection: ECL with 15 second exposure.
ab4464 被引用在 2 文献中.
- Faure AJ et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
- Inoue A et al. Loss of ChlR1 helicase in mouse causes lethality due to the accumulation of aneuploid cells generated by cohesion defects and placental malformation. Cell Cycle 6:1646-54 (2007). PubMed: 17611414