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Anti-SA1抗体(ab4457)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (9)

Product price, shipping and contact information

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Immunocytochemistry - Anti-SA1 antibody (ab4457)
  • Western blot - Anti-SA1 antibody (ab4457)

Key features and details

  • Goat polyclonal to SA1
  • Suitable for: WB, ICC
  • Reacts with: Human, Xenopus laevis
  • Isotype: IgG

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概述

  • 产品名称

    Anti-SA1抗体
    参阅全部 SA1 一抗
  • 描述

    山羊多克隆抗体to SA1
  • 宿主

    Goat
  • 经测试应用

    适用于: WB, ICCmore details
  • 种属反应性

    与反应: Human, Xenopus laevis
    预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey
  • 免疫原

    Synthetic peptide corresponding to Human SA1 aa 1150-1200.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7
    Preservative: 0.1% Sodium azide
    Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Chromatid Cohesion
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Chromosome Structure
    • Chromatid Cohesion

相关产品

  • ChIP Related Products

    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

应用

Our Abpromise guarantee covers the use of ab4457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC 1/500 - 1/2000.

靶标

  • 功能

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • 序列相似性

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • 翻译后修饰

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • 细胞定位

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Target information above from: UniProt accession Q8WVM7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 10274 Human
    • Entrez Gene: 399163 Xenopus laevis
    • Omim: 604358 Human
    • SwissProt: Q8WVM7 Human
    • SwissProt: Q9DGN1 Xenopus laevis
    • Unigene: 412586 Human
    • Unigene: 3441 Xenopus laevis
    • 别名

      • Cohesin subunit SA 1 antibody
      • Cohesin subunit SA-1 antibody
      • Cohesin Subunit SA1 antibody
      • Nuclear protein stromal antigen 1 antibody
      • SA 1 antibody
      • SCC 3 homolog 1 antibody
      • Scc3 antibody
      • SCC3 homolog 1 antibody
      • STAG 1 antibody
      • stag1 antibody
      • STAG1_HUMAN antibody
      • Stromal antigen 1 antibody
      see all

    图片

    • Immunocytochemistry - Anti-SA1 antibody (ab4457)
      Immunocytochemistry - Anti-SA1 antibody (ab4457)
      Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.
    • Western blot - Anti-SA1 antibody (ab4457)
      Western blot - Anti-SA1 antibody (ab4457)

      Nuclear Extract from HeLa cells (~5 x 105 cells).

      ab4457 at 0.5 µg/ml.

      Detection: ECL with 15 second exposure.

      Nuclear Extract from HeLa cells (~5 x 105 cells).

      ab4457 at 0.5 µg/ml.

      Detection: ECL with 15 second exposure.

    实验方案

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (9)

    发表研究结果有使用 ab4457?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4457 被引用在 9 文献中.

    • Lovejoy CA  et al. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18:e3000594 (2020). PubMed: 31895940
    • Meisenberg C  et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
    • Steiner LA  et al. CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells. PLoS One 11:e0155378 (2016). ChIP . PubMed: 27219007
    • Uusküla-Reimand L  et al. Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders. Genome Biol 17:182 (2016). WB . PubMed: 27582050
    • Fukuda T  et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). PubMed: 24797475
    • Schmidt D  et al. Waves of retrotransposon expansion remodel genome organization and CTCF binding in multiple mammalian lineages. Cell 148:335-48 (2012). PubMed: 22244452
    • Faure AJ  et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
    • Schmidt D  et al. A CTCF-independent role for cohesin in tissue-specific transcription. Genome Res 20:578-88 (2010). ChIP ; Human . PubMed: 20219941
    • Rubio ED  et al. CTCF physically links cohesin to chromatin. Proc Natl Acad Sci U S A 105:8309-14 (2008). ChIP, ChIP/Chip ; Human . PubMed: 18550811

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-3 of 3 Abreviews or Q&A

    Question

    It states that the immunogen used was a synthetic peptide (Human) - 35 amino acids which represented a portion of SA 1 encoded in part by exons 35 and 36. However, the customer says that there are only 34 exons. Please explain more about the immunogen and where the information is stating there are exons 35 and 36.

    Read More

    Abcam community

    Verified customer

    Asked on May 09 2006

    Answer

    The epitope recognized by ab4457 maps to a region between residues 1150 and 1200 of human Stromal Antigen 1 using the numbering given in entry NP_005853.2 (GeneID 10274). I hope this helps, please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 May 10 2006

    Question

    Ok thanks. I would like to order as soon as possible but I am not sure how your company normally test the use of antibodies for ICC and how you can be sure that the signal you are getting is specific. So, the information would be of great help.

    Read More

    Abcam community

    Verified customer

    Asked on Jun 17 2004

    Answer

    Thank you very much for your patience. I'm afraid that I am unable to get this information from the originator of ab4455 and ab4457. I apologize for the inconvenience and if I can be of further assistance, please do let me know.

    Read More

    Abcam Scientific Support

    回复于 Jul 07 2004

    Question

    I have a question related to the four types of goat anti-SCC3 antibodies that you have (Ab4455, Ab4457, Ab4463, and Ab4464). Do you know which one works best on immunostaining?

    Read More

    Abcam community

    Verified customer

    Asked on May 05 2004

    Answer

    Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    回复于 May 06 2004

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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