Anti-SA1抗体(ab4457)
Key features and details
- Goat polyclonal to SA1
- Suitable for: WB, ICC, IP
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SA1抗体
参阅全部 SA1 一抗 -
描述
山羊多克隆抗体to SA1 -
宿主
Goat -
经测试应用
适用于: WB, ICC, IPmore details -
种属反应性
与反应: Human
预测可用于: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey
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免疫原
Synthetic peptide corresponding to Human SA1 aa 1150-1200.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
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| ICC |
1/500 - 1/2000.
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| IP |
Use at 2-10 µg/mg of lysate.
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| 说明 |
|---|
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WB
1/2000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa). |
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ICC
1/500 - 1/2000. |
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IP
Use at 2-10 µg/mg of lysate. |
靶标
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功能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
序列相似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻译后修饰
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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数据库链接
- Entrez Gene: 10274 Human
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- Unigene: 412586 Human
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别名
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
图片
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Western blot - Anti-SA1 antibody (ab4457)All lanes : Anti-SA1 antibody (ab4457) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : 293T Whole cell lysate
Lane 3 : Jurkat Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 144 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunoprecipitation - Anti-SA1 antibody (ab4457)SA1 was immunoprecipitated from HeLa whole cell lysate with 6 µg/mg lysate ab4457. Western blot was performed from the immunoprecipitate using ab4457 at 0.1 µg/ml dilution.
Lane 1: ab4457 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
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Immunocytochemistry - Anti-SA1 antibody (ab4457)Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4457 at 1µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab4457 被引用在 10 文献中.
- Lovejoy CA et al. ATRX affects the repair of telomeric DSBs by promoting cohesion and a DAXX-dependent activity. PLoS Biol 18:e3000594 (2020). PubMed: 31895940
- Nora EP et al. Molecular basis of CTCF binding polarity in genome folding. Nat Commun 11:5612 (2020). PubMed: 33154377
- Meisenberg C et al. Repression of Transcription at DNA Breaks Requires Cohesin throughout Interphase and Prevents Genome Instability. Mol Cell 73:212-223.e7 (2019). PubMed: 30554942
- Steiner LA et al. CTCF and CohesinSA-1 Mark Active Promoters and Boundaries of Repressive Chromatin Domains in Primary Human Erythroid Cells. PLoS One 11:e0155378 (2016). ChIP . PubMed: 27219007
- Uusküla-Reimand L et al. Topoisomerase II beta interacts with cohesin and CTCF at topological domain borders. Genome Biol 17:182 (2016). WB . PubMed: 27582050
- Fukuda T et al. STAG3-mediated stabilization of REC8 cohesin complexes promotes chromosome synapsis during meiosis. EMBO J 33:1243-55 (2014). PubMed: 24797475
- Schmidt D et al. Waves of retrotransposon expansion remodel genome organization and CTCF binding in multiple mammalian lineages. Cell 148:335-48 (2012). PubMed: 22244452
- Faure AJ et al. Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. Genome Res 22:2163-75 (2012). ChIP-sequencing ; Mouse . PubMed: 22780989
- Schmidt D et al. A CTCF-independent role for cohesin in tissue-specific transcription. Genome Res 20:578-88 (2010). ChIP ; Human . PubMed: 20219941
- Rubio ED et al. CTCF physically links cohesin to chromatin. Proc Natl Acad Sci U S A 105:8309-14 (2008). ChIP, ChIP/Chip ; Human . PubMed: 18550811