Anti-SA1抗体(ab4455)
Key features and details
- Goat polyclonal to SA1
- Suitable for: IP, WB, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-SA1抗体
参阅全部 SA1 一抗 -
描述
山羊多克隆抗体to SA1 -
宿主
Goat -
经测试应用
适用于: IP, WB, ICCmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rhesus monkey -
免疫原
Synthetic peptide within Human SA1 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements. NP_005853.2 (GeneID 10274).
Database link: Q8WVM7 -
阳性对照
- WB: HeLa nuclear extract. HeLa, MCF-7, HEK-293T, TCMK-1 and NIH/3T3 whole cell lysate. ICC: HeLa cells. IP: SA1 IP in HeLa whole cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
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ICC |
1/500 - 1/2000.
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说明 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa). |
ICC
1/500 - 1/2000. |
靶标
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功能
Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. -
序列相似性
Belongs to the SCC3 family.
Contains 1 SCD (stromalin conservative) domain. -
翻译后修饰
Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation. -
细胞定位
Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. - Information by UniProt
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数据库链接
- Entrez Gene: 10274 Human
- Entrez Gene: 20842 Mouse
- Omim: 604358 Human
- SwissProt: Q8WVM7 Human
- SwissProt: Q9D3E6 Mouse
- Unigene: 412586 Human
- Unigene: 42135 Mouse
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别名
- Cohesin subunit SA 1 antibody
- Cohesin subunit SA-1 antibody
- Cohesin Subunit SA1 antibody
see all
图片
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Whole cell lysate (15 µg) from HeLa, MCF-7, HEK-293T, TCMK-1, and NIH/3T3 cells prepared using NETN lysis buffer.
ab4455 used for WB at 0.1 µg/ml.
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Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Lane 1: ab4455 used for IP at 6 µg per reaction.
Lane 2: Control IgG.
For blotting immunoprecipitated SA1, ab4455 was used at 0.1 µg/ml.
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Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5
µ g/ml.Detection: ECL with 15 second exposure.
Nuclear Extract from HeLa cells (~5 x 105 cells).
ab4455 at 0.5 µg/ml.
Detection: ECL with 15 second exposure. -
Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab4455 被引用在 4 文献中.
- Tian H et al. EWSR1 affects PRDM9-dependent histone 3 methylation and provides a link between recombination hotspots and the chromosome axis protein REC8. Mol Biol Cell 32:1-14 (2021). PubMed: 33175657
- Hopkins J et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). WB ; Mouse . PubMed: 24992337
- Xiao T et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . PubMed: 21444719
- Leman AR et al. Human Timeless and Tipin stabilize replication forks and facilitate sister-chromatid cohesion. J Cell Sci 123:660-70 (2010). WB, IP ; Human . PubMed: 20124417