概述

  • 产品名称
  • 描述
    兔多克隆抗体to S6K1
  • 宿主
    Rabbit
  • 特异性
    Reacts strongly with 70 kd of p70 S6K, but only weakly with the 80 kDa S6K.
  • 经测试应用
    适用于: WB, ELISA, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Rabbit, Cow, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to Human S6K1 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    ISKRPEHLRMNL

  • 阳性对照
    • Use Mouse brain extract.

性能

应用

Our Abpromise guarantee covers the use of ab9366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250.
ELISA 1/500.
IP Use at 3 µg/mg of lysate.

靶标

  • 功能
    Acts to integrate nutrient and growth factor signals in regulation of protein synthesis, cell proliferation, cell growth, cell cycle progression and cell survival. Downstream effector of the mTOR signaling pathway. Phosphorylates specifically ribosomal protein S6 in response to insulin or several classes of mitogens. During translation initiation, the inactive form associatess with the eIF-3 complex under conditions of nutrient depletion. Mitogenic stimulation leads to phosphorylation and dissociation from the eIF-3 complex and the free activated form can phosphorylate other translational targets including EIF4B. Promotes protein synthesis by phosphorylating PDCD4 at 'Ser-67' and targeting it for degradation. Phosphorylates RICTOR leading to regulation of mammalian target of rapamycin complex 2 (mTORC2) signaling; probably phosphorylates RICTOR at 'Thr-1135'. Phosphorylates IRS1 at multiple serine residues coupled with insulin resistance; probably phosphorylates IRS1 at 'Ser-270'. Required for TNF-alpha induced IRS-1 degradation. Phosphorylates EEF2K in response to IGF1 and inhibits EEF2K activity. Phosphorylates BAD at 'Ser-99' in response to IGF1 leading to BAD inactivation and inhibition of BAD-induced apoptosis. Phosphorylates mitochondrial RMP leading to dissociation of a RMP:PPP1CC complex; probably phosphorylates RMP at 'Ser-99'. The free mitochondrial PPP1CC can dephosphorylate RPS6KB1 at Thr-412 which is proposed to be a negative feed back mechanism for the RPS6KB1 antiapoptotic function. Phosphorylates GSK3B at 'Ser-9' under conditions leading to loss of the TSC1-TSC2 complex. Phosphorylates POLDIP3.
  • 组织特异性
    Widely expressed.
  • 序列相似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. S6 kinase subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 结构域
    The autoinhibitory domain is believed to block phosphorylation within the AGC-kinase C-terminal domain and the activation loop.
    The TOS (TOR signaling) motif is essential for activation by mTORC1.
  • 翻译后修饰
    Phosphorylation at Thr-412 is regulated by mTORC1. The phosphorylation at this site is maintained by an agonist-dependent autophosphorylation mechanism.
  • 细胞定位
    Cytoplasm; Nucleus. Cytoplasm and Cell junction > synapse > synaptosome. Mitochondrion outer membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 70 kDa ribosomal protein S6 kinase 1 antibody
    • KS6B1_HUMAN antibody
    • p70 alpha antibody
    • P70 beta 1 antibody
    • p70 ribosomal S6 kinase alpha antibody
    • p70 ribosomal S6 kinase beta 1 antibody
    • p70 S6 kinase alpha antibody
    • P70 S6 Kinase antibody
    • p70 S6 kinase, alpha 1 antibody
    • p70 S6 kinase, alpha 2 antibody
    • p70 S6K antibody
    • p70 S6K-alpha antibody
    • p70 S6KA antibody
    • p70(S6K) alpha antibody
    • p70(S6K)-alpha antibody
    • p70-alpha antibody
    • p70-S6K 1 antibody
    • p70-S6K antibody
    • P70S6K antibody
    • P70S6K1 antibody
    • p70S6Kb antibody
    • PS6K antibody
    • Ribosomal protein S6 kinase 70kDa polypeptide 1 antibody
    • Ribosomal protein S6 kinase beta 1 antibody
    • Ribosomal protein S6 kinase beta-1 antibody
    • Ribosomal protein S6 kinase I antibody
    • RPS6KB1 antibody
    • S6K antibody
    • S6K-beta-1 antibody
    • S6K1 antibody
    • Serine/threonine kinase 14 alpha antibody
    • Serine/threonine-protein kinase 14A antibody
    • STK14A antibody
    see all

文献

This product has been referenced in:
  • Amara S  et al. Critical role of SIK3 in mediating high salt and IL-17 synergy leading to breast cancer cell proliferation. PLoS One 12:e0180097 (2017). Read more (PubMed: 28658303) »
  • Feng F  et al. Hoxa5 increases mitochondrial apoptosis by inhibiting Akt/mTORC1/S6K1 pathway in mice white adipocytes. Oncotarget 8:95332-95345 (2017). Read more (PubMed: 29221131) »

See all 9 Publications for this product

客户评价及客户问答

Thank you for contacting us.

Yes the customer would be eligible for a testing discount for ab12864 if they are willing to test it in mouse and/or sheep samples with western blotting. The sequence surrounding the Serine 235 and 236 of human RP...

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Vielen Dank für Ihre Anfrage und dafür, dass Sie sich die Zeit genommen haben, unseren Fragebogen auszufüllen. Es tut mir leid, dass Sie Probleme mit unserem Antikörper hatten. Ich habe gesehen, dass Sie schon mit meiner Kollegin be...

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I can now confirm the discount code is xxxxx for which the expiration date is the 24th of July 2012. In order to activate the code, please submit an Abreview for ab12864 using mouse and/or sheep sa...

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I understand your concerns over the antibody. I suggested that the antibody may be at fault not because we have had other complaints from customers but because of the optimisation having been performed by the...

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Thank you for taking time to complete our questionnaire and for providing me with that extra information. I am sorry that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and w...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results. We take customer complaints very seriously and investigate every antibody we feel may not be performing as expected. T...

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Thank you very much for your interest in ab9366.

To our knowledge, ab9366 has not been tested in IHC. Therefore, I can offer a discount off a future purchase if you buy ab9366 now, test it in IHC and submit feedback to us in the form of an A...

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Unfortunately, we are not specialists in this area and we do not want to recommend a product that is unsuitable for your needs. We suggest that you refer to the latest literature in this field.

This antibody has been succesfully tested in the listed applications using samples from mice. I am sorry but we can't reveal the name of the vendor, as this is commercially sensitive information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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