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Cardiovascular Angiogenesis Angiogenic Factors
使用敲除细胞株进行验证重组RabMAb

重组Anti-S100A4抗体[EPR14639(2)] (ab197896)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (4) Submit a question References (19)

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Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Flow Cytometry - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
  • Anti-S100A4 antibody [EPR14639(2)] (ab197896)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14639(2)] to S100A4
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-S100A4抗体[EPR14639(2)]
    参阅全部 S100A4 一抗
  • 描述

    兔单克隆抗体[EPR14639(2)] to S100A4
  • 宿主

    Rabbit
  • 特异性

    Based on sequence homologies, the antibody may cross-react with other proteins of the same family (S100A1-12). We did not perform any experiments to confirm this.

    We do not guarantee IHC-P for mouse and rat. Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.  Please see images below for suitable positive controls.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: A549, A375, HeLa, NIH/3T3, Raw264.7 cell lysates. Human fetal spleen, colon and lung tissue lysates. Mouse spleen, bone marrow and heart tissues lysates. IHC-P: Human cervix carcinoma, lung carcinoma and gastric carcinoma tissues, rat spleen tissue. ICC/IF: HeLa cells. Flow Cyt: Jurkat cells.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    EPR14639(2)
  • 同种型

    IgG
  • 研究领域

    • Cardiovascular
    • Angiogenesis
    • Angiogenic Factors
    • Signal Transduction
    • Signaling Pathway
    • Calcium Signaling
    • Calcium Binding Proteins
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Myocardial regeneration

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-S100A4 antibody [EPR14639(2)] (ab208566)
    • Alexa Fluor® 555 Anti-S100A4 antibody [EPR14639(2)] (ab210670)
    • Anti-S100A4 antibody [EPR14639(2)] - BSA and Azide free (ab220213)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human S100A4 knockout HeLa cell line (ab261758)
  • Recombinant Protein

    • Recombinant Human S100A4 protein (ab85489)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab197896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Rat
Human
IP
Human
WB
Mouse
Human
应用 Ab评论 说明
IHC-P (1)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF (2)
1/250.
IP
1/40.
Flow Cyt
1/250.
说明
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

We do not guarantee IHC-P for mouse and rat.

WB
1/1000. Detects a band of approximately 12 kDa (predicted molecular weight: 12 kDa).
ICC/IF
1/250.
IP
1/40.
Flow Cyt
1/250.

靶标

  • 组织特异性

    Ubiquitously expressed.
  • 序列相似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • Target information above from: UniProt accession P26447 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6275 Human
    • Entrez Gene: 20198 Mouse
    • Entrez Gene: 24615 Rat
    • Omim: 114210 Human
    • SwissProt: P26447 Human
    • SwissProt: P07091 Mouse
    • SwissProt: P05942 Rat
    • Unigene: 654444 Human
    • Unigene: 3925 Mouse
    • Unigene: 504 Rat
    see all
  • 别名

    • 18A2 antibody
    • 42A antibody
    • calcium Placental protein antibody
    • Calvasculin antibody
    • CAPL antibody
    • Fibroblast specific protein 1 (FSP1) antibody
    • Fibroblast specific protein 1 antibody
    • Fibroblast specific protein antibody
    • FSP1 antibody
    • Leukemia multidrug resistance associated protein antibody
    • Malignant transformation suppression 1 (MTS1) antibody
    • Malignant transformation suppression 1 antibody
    • Metastasin antibody
    • MTS1 antibody
    • OTTHUMP00000015467 antibody
    • OTTHUMP00000015468 antibody
    • P9KA antibody
    • PEL98 antibody
    • Placental calcium-binding protein antibody
    • Protein Mts1 antibody
    • Protein S100 A4 antibody
    • Protein S100-A4 antibody
    • S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog) antibody
    • S100 calcium binding protein A4 antibody
    • S100 calcium-binding protein A4 antibody
    • S100a4 antibody
    • S10A4_HUMAN antibody
    see all

图片

  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : S100A4 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab197896 observed at 12 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab197896 Recombinant Anti-S100A4 antibody [EPR14639(2)] was shown to specifically react with S100A4 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261758 (knockout cell lysate ab257045) was used. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. ab197896 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lane 1 : Wild-type A549 whole cell lysate
    Lane 2 : S100A4 knockout A549 whole cell lysate
    Lane 3 : A375 whole cell lysate
    Lane 4 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 12 kDa
    Observed band size: 12 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab197896 observed at 12 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab197896 was shown to recognize S100A4 in wild-type A549 cells as signal was lost at the expected MW in S100A4 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and S100A4 knockout samples were subjected to SDS-PAGE. Ab197896 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling S100A4 with ab197896 at 1/2000 dilution (0.376 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on lymphocytes of rat spleen.The section was incubated with ab197896 for 30 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

  • Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunocytochemistry/ Immunofluorescence - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling S100A4 with ab197896 at 1/200 (10 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% TritonX-100. ab150077, AlexaFluor®488 Goat anti-Rabbit at 1/1000 (2 μg/mL) was used as the secondary antibody. Cells were counterstained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/mL). Nuclear counter stain was DAPI (blue). 
     
    Confocal image showing positive staining in HeLa cell line.
  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lanes 1 & 5 : A549 whole cell lysate
    Lane 2 : Human liver lysates
    Lane 3 : Human lung lysates
    Lane 4 : Human colon lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa


    Exposure time: 26 seconds
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at 1/500 dilution. Cells were counterstained with Hematoxylin. 

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Cytoplasm and nuclear staining on human gastric carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lane 1 : A375 (human malignant melanoma) whole cell lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 12 kDa


    Exposure time: 3 minutes


    Blocking/dilution buffer: 5% NFDM/TBST

  • Flow Cytometry - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Flow Cytometry - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    Flow Cytometry analysis of Jurkat cells labelling S100A4 with ab197896 at 1/250 (red). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunoprecipitation - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    ab197896 at 1/40 immunoprecipitating S100A4 in A549 whole cell lysate observed at 12 KDa.

    Lane 1 (input): A549 whole cell lysate 10μg

    Lane 2 (+): ab197896 + A549 whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab197896 in A549 whole cell lysate

    For western blotting, Panel A: ab197896, 1:1000; Panel B: ab124805, 1:1000 and anti-rabbit IgG (HRP), specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used ay 1/500 dilution as a secondary antibody and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and cytoplasm staining on cervix carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100A4 antibody [EPR14639(2)] (ab197896)

    Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling S100A4 using ab197896 at 1/2000 dilution. Goat Anti-Rabbit IgG H&L (HRP) ab97051 was used as a secondary antibody at a dilution of 1/500 and cells were counterstained with Hematoxylin.

    Inset image: negative control obtained using PBS instead of ab197896 and secondary antibody only.
    Note: Nuclear and weakly staining on lung carcinoma tissue was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Western blot - Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    All lanes : Anti-S100A4 antibody [EPR14639(2)] (ab197896) at 1/1000 dilution

    Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : Raw264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
    Lane 4 : Mouse spleen tissue lysates
    Lane 5 : Mouse bone marrow tissue lysates
    Lane 6 : Mouse heart tissue lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa
    Observed band size: 12 kDa


    Exposure time: 44 seconds


    Blocking and dilution buffer: 5% NFDM/TBST.

    Some optimisation may be required for detection of the target protein due to low levels of endogenous expression in some samples.

  • Anti-S100A4 antibody [EPR14639(2)] (ab197896)
    Anti-S100A4 antibody [EPR14639(2)] (ab197896)

实验方案

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

文献 (19)

发表研究结果有使用 ab197896?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab197896 被引用在 19 文献中.

  • Cai X  et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
  • Cai Y  et al. Elimination of senescent cells by ß-galactosidase-targeted prodrug attenuates inflammation and restores physical function in aged mice. Cell Res N/A:N/A (2020). PubMed: 32341413
  • Dhall S  et al. A Flowable Placental Formulation Prevents Bleomycin-Induced Dermal Fibrosis in Aged Mice. Int J Mol Sci 21:N/A (2020). PubMed: 32545915
  • Yin Y  et al. Tongxinluo Attenuates Myocardiac Fibrosis after Acute Myocardial Infarction in Rats via Inhibition of Endothelial-to-Mesenchymal Transition. Biomed Res Int 2019:6595437 (2019). PubMed: 31317035
  • Liu ZH  et al. SIRT1 activation attenuates cardiac fibrosis by endothelial-to-mesenchymal transition. Biomed Pharmacother 118:109227 (2019). PubMed: 31351433
View all Publications for this product

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1-4 of 4 Abreviews

Immunocytochemistry abreview for Anti-S100A4 antibody [EPR14639(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Dog Cultured Cells (Dermal fibroblast)
Permeabilization
Yes - 0.1% Triton X-100
Specification
Dermal fibroblast
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Jun 01 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-S100A4 antibody [EPR14639(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (H9c2 rat cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
H9c2 rat cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 18 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-S100A4 antibody [EPR14639(2)]

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Cardiomyocytes)
Permeabilization
Yes - 0.01% TritonX-100
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

提交于 Mar 09 2020

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100A4 antibody [EPR14639(2)]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Fixative
Paraformaldehyde
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