重组Anti-S100A11抗体[EPR11172] (ab180593)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11172] to S100A11
- Suitable for: Flow Cyt (Intra), IHC-P, ICC/IF
- Reacts with: Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-S100A11抗体[EPR11172]
参阅全部 S100A11 一抗 -
描述
兔单克隆抗体[EPR11172] to S100A11 -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), IHC-P, ICC/IFmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- IHC-P: Human ovarian carcinoma tissue and human thyroid carcinoma. ICC/IF: BxPC-3 and PC-3 cells. Flow Cyt (intra): PC-3 and PC-12 cells.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.21% BSA, 59% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11172 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab180593于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/80 - 1/150.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
IHC-P | (1) |
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Unpurified format: 1/50 - 1/100. For anitgen retireval heat up to 98°C, below boiling, and then let cool for 10-20 min. |
ICC/IF |
1/100 - 1/400.
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说明 |
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Flow Cyt (Intra)
1/80 - 1/150. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
IHC-P
1/3000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Unpurified format: 1/50 - 1/100. For anitgen retireval heat up to 98°C, below boiling, and then let cool for 10-20 min. |
ICC/IF
1/100 - 1/400. |
靶标
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功能
Facilitates the differentiation and the cornification of keratinocytes. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
翻译后修饰
Phosphorylation at Thr-10 by PRKCA significantly suppresses homodimerization and promotes association with NCL/nucleolin which induces nuclear translocation. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 6282 Human
- Entrez Gene: 445415 Rat
- Omim: 603114 Human
- SwissProt: P31949 Human
- SwissProt: Q6B345 Rat
- Unigene: 417004 Human
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别名
- Calgizzarin antibody
- Epididymis secretory protein Li 43 antibody
- HEL S 43 antibody
see all
图片
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ab180593 staining S100A11 in human thyroid carcinoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraffin and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/3000. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.
Negative control 1: PBS in place of primary antibody.
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Intracellular Flow Cytometry analysis of PC-3 cells using ab180593 at a 1/150 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.
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Immunofluorescence analysis of BxPC-3 cells (fixative 4% paraformaldehyde) labeling S100A11 with ab180593 at a 1/100 dilution (left image), and counterstained with Dapi (right image). Goat anti rabbit IgG (Dylight 555) secondary used at a 1/200 diution.
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ab180593 staining S100A11 in PC-3 (human prostate adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/400. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab180593 and secondary antibody ab150077 respectively and DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
ab180593 staining S100A11 in PC-12 (rat adrenal gland pheochromocytoma) cell line by intracellular flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/180. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
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Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue labeling S100A11 with ab180593 at a 1/100 dilution. Prediluted ImmunoHistoprobe HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (6)
ab180593 被引用在 6 文献中.
- Tu Y et al. S100A11 functions as novel oncogene in glioblastoma via S100A11/ANXA2/NF-?B positive feedback loop. J Cell Mol Med 23:6907-6918 (2019). PubMed: 31430050
- Xia Q et al. S100A11 protects against neuronal cell apoptosis induced by cerebral ischemia via inhibiting the nuclear translocation of annexin A1. Cell Death Dis 9:657 (2018). PubMed: 29844306
- Jover B et al. Sodium restriction modulates innate immunity and prevents cardiac remodeling in a rat model of metabolic syndrome. Biochim Biophys Acta Mol Basis Dis 1863:1568-1574 (2017). PubMed: 28254494
- Andrés Cerezo L et al. Calgizzarin (S100A11): a novel inflammatory mediator associated with disease activity of rheumatoid arthritis. Arthritis Res Ther 19:79 (2017). WB, IHC-P ; Human . PubMed: 28446208
- Advedissian T et al. E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface. Sci Rep 7:17086 (2017). PubMed: 29213102
- Gocheva V et al. Quantitative proteomics identify Tenascin-C as a promoter of lung cancer progression and contributor to a signature prognostic of patient survival. Proc Natl Acad Sci U S A 114:E5625-E5634 (2017). PubMed: 28652369