Anti-S100抗体，prediluted (ab948)
Key features and details
- Rabbit polyclonal to S100, prediluted
- Suitable for: IHC-P
- Reacts with: Cow, Human
- Isotype: IgG
概述
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产品名称
Anti-S100抗体，prediluted
参阅全部 S100 一抗 -
描述
兔多克隆抗体to S100，prediluted -
宿主
Rabbit -
特异性
This antibody reacts with cow S-100 protein A and B. It shows strong cross reactivity with human S-100 A and B. In the brain, it labels glial and ependymal cells. Melanocytes and Langerhans cells of the skin are also stained with this antibody. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Cow, Human -
免疫原
Affinity purified S-100 protein isolated from cow brain.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
Preservative: 0.05% Sodium azide
Inert stabilizer -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
骨髓瘤
unknown -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | Use a concentration of 4 µg/ml. This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections. No further titration is required. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions should be determined by the user. Proteolytic digestion of tissue section is required prior to staining. |
靶标
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功能
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
组织特异性
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
序列相似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 431787 Cow
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P02639 Cow
- SwissProt: P23297 Human
- Unigene: 515715 Human
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别名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody, prediluted (ab948)
ab948 staining human normal skin. Staining is localised to nuclear and cytoplasmic compartments.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus, at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffer citrate pH6 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend individual optmisation of all of the forementioned parameters.
文献 (2)
ab948 被引用在 2 文献中.
- Ding J et al. Long non-coding RNA MIF-AS1 promotes breast cancer cell proliferation, migration and EMT process through regulating miR-1249-3p/HOXB8 axis. Pathol Res Pract 215:152376 (2019). PubMed: 31097355
- Ding L et al. GABA(A) receptor alpha1 subunit mutation A322D associated with autosomal dominant juvenile myoclonic epilepsy reduces the expression and alters the composition of wild type GABA(A) receptors. J Biol Chem 285:26390-405 (2010). PubMed: 20551311
