Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH6
Permeabilization
No
Specification
Cerebellum
Blocking step
Normal Donkey Serum (NDS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: PBS with 2% NDS and 1% Triton X-100
Dilution
1/200
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: Anti-mouse antibody (Jackson 715-545-150)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Figure. IHC and confocal microscopy for S100 in the white matter of cerebellum from an adult male Wistar rat. A-B: Immunolabeling for S100. Astrocytes express S100 (magenta), in their somas and projections (green arrows). Nuclei (Nu) were counterstained with DAPI (grey). C: 3D projection of the astrocytes pointed in A and B. Image generated at 60X with 2.5 zoom, in an Olympus FV1000 confocal microscope. Authors: Martin Avila, Leandro Freites and Estela Muñoz (IHEM-UNCuyo-CONICET, Mendoza, Argentina).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Estela Munoz
Verified customer
提交于 Mar 15 2021