Name: Anti-S100 antibody [4C4.9]
SKU: ab4066
Rating: 4 (12 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Key features and details

Mouse monoclonal [4C4.9] to S100
Suitable for: IHC-P
Reacts with: Human
Isotype: IgG2a

产品名称

Anti-S100抗体[4C4.9]
参阅全部 S100 一抗
描述

小鼠单克隆抗体[4C4.9] to S100
宿主

Mouse
经测试应用

适用于: IHC-Pmore details
种属反应性

与反应: Human
免疫原

Full length protein. Purified bovine brain S-100 protein
阳性对照
- IHC-P: Human Melanoma and skin tissue.
常规说明

This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.3
Preservative: 0.05% Sodium azide
Constituent: Tissue culture supernatant
Concentration information loading...
纯度

Tissue culture supernatant
克隆

单克隆
克隆编号

4C4.9
同种型

IgG2a
研究领域

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

The Abpromise guarantee

Abpromise™承诺保证使用ab4066于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P	(4)	1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

说明
IHC-P 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

功能

Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
组织特异性

Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
序列相似性

Belongs to the S-100 family.
Contains 2 EF-hand domains.
细胞定位

Cytoplasm.
Target information above from: UniProt accession P23297 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
别名
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
- S-100 protein alpha chain antibody
- S-100 protein subunit alpha antibody
- S100 alpha antibody
- S100 beta antibody
- S100 calcium binding protein A1 antibody
- S100 calcium binding protein B antibody
- S100 calcium-binding protein A1 antibody
- S100 protein alpha polypeptide antibody
- S100A antibody
- s100a1 antibody
- S100B antibody
- S100beta antibody
- S10A1_HUMAN antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

This image was generated using the ascites version of the product.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.

This image was generated using the ascites version of the product.

IHC-FoFr protocol

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab4066？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4066 被引用在 66 文献中.

Qu H et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
Hernández-Díaz N et al. Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals. J Exp Biol 224:N/A (2021). PubMed: 33568441
Wang Y et al. Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Mol Med 27:27 (2021). PubMed: 33794764
Omiya H et al. BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors. Sci Rep 11:613 (2021). PubMed: 33436697
Rinwa P et al. Demise of nociceptive Schwann cells causes nerve retraction and pain hyperalgesia. Pain 162:1816-1827 (2021). PubMed: 33979318

View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A

提交评价提交问题

1-10 of 23 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Dog Tissue sections (Melanoma suspicious cutaneous tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Tris/EDTA

Permeabilization

Yes - H2O2

Specification

Melanoma suspicious cutaneous tissue

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Apr 25 2022

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rat Tissue sections (Cerebellum)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH6

Permeabilization

Specification

Cerebellum

Blocking step

Normal Donkey Serum (NDS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Fixative

Paraformaldehyde

DR. Estela Munoz

Verified customer

提交于 Mar 15 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (melanoma)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0

Permeabilization

Specification

melanoma

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Aug 19 2016

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (skin)

Antigen retrieval step

Other

Permeabilization

Specification

skin

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Formaldehyde

Abcam user community

Verified customer

提交于 Dec 14 2015

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Brain (Hippocampus))

Antigen retrieval step

None

Permeabilization

Yes - 0.1%TritonX-100/PBS

Specification

Brain (Hippocampus)

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 May 14 2015

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (skin)

Specification

skin

Fixative

Acetone

Permeabilization

Yes - 0,1% Saponin / PBS

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Feb 27 2012

Question

IHC-FoFr with rat peripheral nerve
sees no staining
fix: 4% PFA perfused, postfixed with PFA
no AR
Ab: 1/100 o/n 4 degree; 1/50 o/n RT
block: BSA + goat serum
2nd: goat anti-mouse, OK

suggested: try an enzymatic AR step

Abcam community

Verified customer

Asked on Feb 21 2013

Answer

As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

If not, please let me know.

As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

Abcam Scientific Support

回复于 Feb 21 2013

Question

Dear Sir or Madam,

could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

Best Regards,

Abcam community

Verified customer

Asked on Aug 10 2012

Answer

Thank you for your interest in our products.

The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

If I can assist further, please do not hesitate to contact me.

Abcam Scientific Support

回复于 Aug 10 2012

Question

Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

Abcam community

Verified customer

Asked on May 03 2012

Answer

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

Abcam Scientific Support

回复于 May 03 2012

Question

I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

Abcam community

Verified customer

Asked on Apr 30 2012

Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Abcam Scientific Support

回复于 Apr 30 2012

1-10 of 23 Abreviews or Q&A

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Compatible Secondaries

Isotype control

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (66)

客户评价及客户问答

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker