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Neuroscience Neurotransmission Calcium Signaling Calcium Binding Proteins

Anti-S100抗体[4C4.9] (ab4066)

  • Datasheet
  • SDS
Reviews (10)Q&A (11)References (47)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Key features and details

  • Mouse monoclonal [4C4.9] to S100
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

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概述

  • 产品名称

    Anti-S100抗体[4C4.9]
    参阅全部 S100 一抗
  • 描述

    小鼠单克隆抗体[4C4.9] to S100
  • 宿主

    Mouse
  • 经测试应用

    适用于: IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Full length protein. Purified bovine brain S-100 protein

  • 阳性对照

    • IHC-P: Human Melanoma and skin tissue.
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: Ascites, 1% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • 克隆

    单克隆
  • 克隆编号

    4C4.9
  • 同种型

    IgG2a
  • 研究领域

    • Neuroscience
    • Neurotransmission
    • Calcium Signaling
    • Calcium Binding Proteins
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Astrocyte marker
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class IV
    • Neurofilaments
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)

应用

Our Abpromise guarantee covers the use of ab4066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

靶标

  • 功能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
  • 组织特异性

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
  • 序列相似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P23297 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 6271 Human
    • Omim: 176940 Human
    • SwissProt: P23297 Human
    • Unigene: 515715 Human
    • 别名

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 beta antibody
      • S100 calcium binding protein A1 antibody
      • S100 calcium binding protein B antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S100B antibody
      • S100beta antibody
      • S10A1_HUMAN antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.

    实验方案

    • IHC-FoFr protocol

    Click here to view the general protocols

    数据表及文件

    • Datasheet
    • SDS
  • 文献 (47)

    发表研究结果有使用 ab4066?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4066 被引用在 47 文献中.

    • Zhao W  et al. Expression of P2X receptors in the rat anterior pituitary. Purinergic Signal 16:17-28 (2020). PubMed: 31823189
    • Alanazi AM  et al. Liposomal Resveratrol and/or Carvedilol Attenuate Doxorubicin-Induced Cardiotoxicity by Modulating Inflammation, Oxidative Stress and S100A1 in Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32079097
    • Scherer A  et al. Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs. Genes (Basel) 11:N/A (2020). PubMed: 32456131
    • Goodman T  et al. Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus. Development 147:N/A (2020). PubMed: 32661019
    • Liu J  et al. Embryonic stem cell microenvironment enhances proliferation of human retinal pigment epithelium cells by activating the PI3K signaling pathway. Stem Cell Res Ther 11:411 (2020). PubMed: 32967731
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 21 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
    Permeabilization
    No
    Specification
    melanoma
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Aug 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Dec 14 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain (Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TritonX-100/PBS
    Specification
    Brain (Hippocampus)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 May 14 2015

    Question

    IHC-FoFr with rat peripheral nerve
    sees no staining
    fix: 4% PFA perfused, postfixed with PFA
    no AR
    Ab: 1/100 o/n 4 degree; 1/50 o/n RT
    block: BSA + goat serum
    2nd: goat anti-mouse, OK

    suggested: try an enzymatic AR step

    Read More

    Abcam community

    Verified customer

    Asked on Feb 21 2013

    Answer


    As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

    If not, please let me know.

    As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

    Read More

    Abcam Scientific Support

    回复于 Feb 21 2013

    Question

    Dear Sir or Madam,

    could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

    Best Regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for your interest in our products.

    The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

    If I can assist further, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    回复于 Aug 10 2012

    Question

    Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2012

    Answer

    That's great news! Thanks so much for letting us know.

    Please feel free to contact me if you need anything further.

    Read More

    Abcam Scientific Support

    回复于 May 03 2012

    Question

    I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 30 2012

    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    Abcam Scientific Support

    回复于 Apr 30 2012

    Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Acetone
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 27 2012

    Question

    Thanks for the antibody. It works fine the immunostaining. But I still can not get any signal in western blot, although it was reported that it should work in western blot.



    Do you have any other recommendations for western blot?

    Read More

    Abcam community

    Verified customer

    Asked on Feb 09 2012

    Answer

    Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuccessful. It is possible that this antibody may be sensitive to milk and may produce better results when using BSA.

    For both ab4066 andab868, you could also try increasing the primary antibody concentration to the range 1:200-1:500 or loading more protein (30-50ug per lane). I hope this helps, please let me know if you need any further assistance.

    Read More

    Abcam Scientific Support

    回复于 Feb 09 2012

    Question

    I am trying to do Western Blot and ICC for s100, a marker for astrocytes. The antibody which I got from Abcam is ab4066. I did not get any signal for WB and very weak signal for ICC. So I wonder what is the problem. If I still can not get any signal after trouble shooting, which antibodies do you recommend for WB and ICC, respectively?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 10 2012

    Answer

    Thank you for contacting us. I am sorry that ab4066 was not producing satisfactory signal in ICC and WB. Our antibody ab868 is rated highly for use in ICC and WB on human, mouse, rat, cow, and pig samples. If you could please provide some additional details about the protocol and samples you are using, I would be happy to assist you further. If ab4066 is not working as stated on the datasheet and you have purchased it in the past six months, I would be happy to send you ab868 as a free of charge replacement. Could you please provide the following information? 1) What is your original order or PO number? 2) What species and sample type were you testing? How were the cells / tissuefixed or lysed? 3) What permeabilization and/ or blocking agents were used? 4) Could you please tell me the dilution and incubation times for the primary and secondary antibodies? 5) It would be very helpful if you could please provide an image of your WB or ICC.

    Read More

    Abcam Scientific Support

    回复于 Jan 10 2012

    1-10 of 21 Abreviews or Q&A

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