Anti-S tag抗体(ab183674)
Key features and details
- Rabbit polyclonal to S tag
- Suitable for: WB, IP
- Reacts with: Species independent
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-S tag抗体
参阅全部 S tag 一抗 -
描述
兔多克隆抗体to S tag -
宿主
Rabbit -
经测试应用
适用于: WB, IPmore details -
种属反应性
与反应: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- S tag transfected 293T cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab183674于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000.
|
|
| IP |
Use at an assay dependent concentration.
Use 2.5µg. |
| 说明 |
|---|
|
WB
1/1000 - 1/10000. |
|
IP
Use at an assay dependent concentration. Use 2.5µg. |
靶标
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相关性
S tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. -
别名
- KETAAAKFERQHMDS tag antibody antibody
- S peptide antibody
- S peptide epitope tag antibody
图片
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Western blot - Anti-S tag antibody (ab183674)All lanes : Anti-S tag antibody (ab183674) at 1/5000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : S tag transfected 293T whole cell lysate
Lysates/proteins at 30 µg per lane.12% SDS-PAGE
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Immunoprecipitation - Anti-S tag antibody (ab183674)S tag antibody ab183674 immunoprecipitates S tag protein in IP experiments. IP Sample: 650 µg whole cell lysate of S tag protein expressing HeLa cells (1), 20 μg whole cell lysate of transfected HeLa cells (2), Control with 2.5 μg of preimmune rabbit IgG (3). Immunoprecipitation of S tag protein by 2.5 μg of ab183674. 12% SDS-PAGE. The immunoprecipitated S tag protein was detected by ab183674 diluted at 1/10000. Anti-rabbit IgG was used as a secondary reagent.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab183674 被引用在 3 文献中.
- Salari S et al. Evaluation of a new live recombinant vaccine against cutaneous leishmaniasis in BALB/c mice. Parasit Vectors 13:415 (2020). PubMed: 32787908
- Li C et al. A SnRK1-ZmRFWD3-Opaque2 Signaling Axis Regulates Diurnal Nitrogen Accumulation in Maize Seeds. Plant Cell 32:2823-2841 (2020). PubMed: 32699171
- Yin Z et al. Histone acetyltransferase MoHat1 acetylates autophagy-related proteins MoAtg3 and MoAtg9 to orchestrate functional appressorium formation and pathogenicity in Magnaporthe oryzae. Autophagy 15:1234-1257 (2019). PubMed: 30776962