Key features and details
- Rabbit polyclonal to RTT106
- Suitable for: WB
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
经测试应用适用于: WBmore details
种属反应性与反应: Saccharomyces cerevisiae
Synthetic peptide corresponding to Saccharomyces cerevisiae RTT106 aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: P40161
- This antibody gave a positive signal in both Wild Type Exponentially growing yeast and W303 Saccromyces cerevisiae.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab130122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 52 kDa).|
功能Histones H3 and H4 chaperone involved in the nucleosome formation and heterochromatin silencing. Involved in regulation of Ty1 transposition.
序列相似性Belongs to the RTT106 family.
- Information by UniProt
- Histone chaperone rtt106 antibody
- Regulator of Ty1 transposition protein 106 antibody
- RT106_YEAST antibody
- rtt106 antibody
All lanes : Anti-RTT106 antibody (ab130122) at 1/250 dilution (Milk blocking 3%)
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : W303 Yeast Lysate Whole Cell Lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa (possible non-specific binding)
Exposure time: 20 minutes
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab130122 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.