- 参考文献 (5)
生物学描述Prototypic agonist for AMPA receptors.
Also available in simple stock solutions (ab146679) - add 1 ml of water to get an exact, ready-to-use concentration.
存放说明Store at Room Temperature. The product can be stored for up to 12 months.
溶解度概述Soluble in water to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Our Abpromise guarantee covers the use of ab120130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with (R,S)-AMPA (ab120130), by ICC/IF. Increase in MEK1 (phospho S298) expression correlates with increased concentration of(R,S)-AMPA, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120130 ((R,S)-AMPA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
This product has been referenced in:
- Kew JN & Kemp JA Ionotropic and metabotropic glutamate receptor structure and pharmacology. Psychopharmacology (Berl) 179:4-29 (2005). Read more (PubMed: 15731895) »
- Stensbøl TB et al. The AMPA receptor binding site: focus on agonists and competitive antagonists. Curr Pharm Des 8:857-72 (2002). Read more (PubMed: 11945136) »
- Krogsgaard-Larsen P et al. New class of glutamate agonist structurally related to ibotenic acid. Nature 284:64-6 (1980). Read more (PubMed: 6101908) »