Key features and details
- Goat polyclonal to RPS27/MPS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 RPS27/MPS1 一抗
经测试应用适用于: WBmore details
预测可用于: Mouse, Rat
常规说明Official Gene Symbol - RPS27. LocusLink ID - 6232 (human); 57294 (mouse); 94266 (rat). Gene Ontology terms - structural constituent of ribosome; RNA binding; zinc ion binding; protein biosynthesis; cell proliferation; signal transduction; ribosome; cytosolic small ribosomal subunit (sensu Eukarya); intracellular.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA
Concentration information loading...
纯度Immunogen affinity purified
纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4385 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
组织特异性Expressed in a wide variety of actively proliferating cells and tumor tissues.
序列相似性Belongs to the ribosomal protein S27e family.
- Information by UniProt
- 2610206D02Rik antibody
- 3200001M24Rik antibody
- 40S ribosomal protein S27 antibody
Anti-RPS27/MPS1 antibody (ab4385) at 0.05 µg/ml + Jurkat lysate (35µg protein in RIPA buffer).
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab4385 被引用在 2 文献中.
- Yang ZY et al. Metallopanstimulin-1 regulates invasion and migration of gastric cancer cells partially through integrin ß4. Carcinogenesis N/A:N/A (2013). PubMed: 23803695
- Shin HS et al. Arginine methylation of ribosomal protein S3 affects ribosome assembly. Biochem Biophys Res Commun 385:273-8 (2009). WB ; Human . PubMed: 19460357