Name: Anti-RPA32/RPA2 antibody [9H8]
SKU: ab2175
Rating: 5 (11 reviews)

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Key features and details

Mouse monoclonal [9H8] to RPA32/RPA2
Suitable for: Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IF
Reacts with: Human
Isotype: IgG1

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一抗 - Mouse Anti-RPA32/RPA2 antibody [9H8] (ab2175)
Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IF

蛋白 - Recombinant Human RPA32/RPA2 protein (ab101216)
SDS-PAGE, MS

二抗 - Goat Anti-Mouse IgG H&L (HRP) (ab205719)
WB, IP, ELISA, IHC-P

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产品名称

Anti-RPA32/RPA2抗体[9H8]
参阅全部 RPA32/RPA2 一抗
描述

小鼠单克隆抗体[9H8] to RPA32/RPA2
宿主

Mouse
经测试应用

适用于: Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IFmore details
种属反应性

与反应: Human
免疫原

Full length protein (Human).
阳性对照
- WB: TE 671, SK N BE and HeLa whole cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.05% Sodium azide
Constituent: 1% BSA
Concentration information loading...
纯度

IgG fraction
克隆

单克隆
克隆编号

9H8
骨髓瘤

unknown
同种型

IgG1
轻链类型

kappa
研究领域

Compatible Secondaries
Isotype control
- Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
- Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
Recombinant Protein
- Recombinant Human RPA32/RPA2 protein (ab101216)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

Our Abpromise guarantee covers the use of ab2175 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
Flow Cyt		Use 1µg for 10⁶ cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB		Use at an assay dependent concentration. Predicted molecular weight: 32 kDa.
IP		Use at an assay dependent concentration.
IHC-P		Use at an assay dependent concentration.
IHC-Fr		Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/50-1/100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature.
ICC/IF		Use at an assay dependent concentration. Used at a dilution of 1/300 for 2 hrs on mouse MEF cells (see Abreview for further details).

功能

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
翻译后修饰

Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
细胞定位

Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
Target information above from: UniProt accession P15927 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 6118 Human
- Omim: 179836 Human
- SwissProt: P15927 Human
- Unigene: 703070 Human
- Unigene: 79411 Human
别名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
- AU020965 antibody
- ik:tdsubc_2g1 antibody
- M(2)21C antibody
- MGC137236 antibody
- OTTHUMP00000004008 antibody
- p32 antibody
- p34 antibody
- RCJMB04_6d17 replication protein A2, 32kDa antibody
- REPA2 antibody
- Replication factor A protein 2 antibody
- Replication protein A 32 kDa subunit antibody
- Replication protein A 32kDa subunit antibody
- Replication protein A 34 kDa subunit antibody
- Replication protein A antibody
- Replication Protein A2 (32kDa) antibody
- Replication protein A2 antibody
- Replication protein A2, 32kDa antibody
- RF-A protein 2 antibody
- Rf-A2 antibody
- RFA antibody
- RFA2_HUMAN antibody
- RP-A p32 antibody
- RP-A p34 antibody
- RP21C antibody
- RPA 2 antibody
- RPA 32 antibody
- RPA antibody
- Rpa2 antibody
- RPA32 antibody
- RPA34 antibody
- RpLP1 antibody
- RpP2 antibody
- xx:tdsubc_2g1 antibody
- zgc:109822 antibody
see all

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an Abreview submitted by Dr Kok-Lung Chan

ab2175 at 1/200 staining human skin fibroblasts by ICC/IF. The cells were treated with 2mM hydroxyurea for 16 hours, formaldehyde fixed and permeabilized with 0.5% Triton X100. They were then incubated with the primary antibody for 1.5 hours at 37°C. A Cy3^® conjugated sheep anti-mouse antibody was used as the secondary.
See Abreview
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB.
Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/ml

Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 32 kDa
Observed band size: 32 kDa
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an anonymous Abreview

Anti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).

ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.

Secondary antibody: anti-mouse Molecular Probes Alexa Fluor^® 488 Conjugated at 1/200 dilution.
See Abreview
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)

Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Flow cytometry protocols
Immunohistochemistry protocols

Click here to view the general protocols

发表研究结果有使用 ab2175？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab2175 被引用在 137 文献中.

Lochab S et al. Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome. Elife 9:N/A (2020). PubMed: 32223892
Warren NJH et al. Differential Sensitivity to CDK2 Inhibition Discriminates the Molecular Mechanisms of CHK1 Inhibitors as Monotherapy or in Combination with the Topoisomerase I Inhibitor SN38. ACS Pharmacol Transl Sci 2:168-182 (2019). PubMed: 32259055
Mohni KN et al. HMCES Maintains Genome Integrity by Shielding Abasic Sites in Single-Strand DNA. Cell 176:144-153.e13 (2019). PubMed: 30554877
Lu X et al. GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival. Nucleic Acids Res 47:10977-10993 (2019). PubMed: 31612207
Panier S et al. SLX4IP Antagonizes Promiscuous BLM Activity during ALT Maintenance. Mol Cell 76:27-43.e11 (2019). PubMed: 31447390

View all Publications for this product

客户评价及客户问答

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1-10 of 11 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Permeabilization

Yes - 0.5% triton X

Specification

HeLa

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Feb 08 2017

Application

ChIP

Sample

Human Cell lysate - whole cell (U2OS)

Negative control

cells without 4OHT treatment and no antibody for IP

Specification

U2OS

Detection step

Real-time PCR

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: fromaldehyde

Positive control

4 hydroxy tamoxifen (4OHT) treated cells (double strand breaks formed)

Dr. Federica Marini

Verified customer

提交于 Dec 02 2016

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (U2OS)

Permeabilization

Yes - 0.5% Triton X-100

Specification

U2OS

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 06 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (breast cancer cell line)

Gel Running Conditions

Reduced Denaturing

Loading amount

10 µg

Treatment

HU 1mM 16hr

Specification

breast cancer cell line

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

提交于 Jul 11 2016

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (8%)

Sample

Human Cell lysate - whole cell (U2OS cells)

Specification

U2OS cells

Treatment

1 ´M CPT

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Remi Buisson

Verified customer

提交于 Dec 03 2014

Application

Immunoprecipitation

Immuno-precipitation step

Protein G

Sample

Human Cell lysate - nuclear (U2OS cells)

Specification

U2OS cells

Total protein in input

200 µg

Dr. Remi Buisson

Verified customer

提交于 Nov 22 2014

Application

ChIP

Detection step

Real-time PCR

Sample

Human Cell lysate - nuclear (hek293t)

Specification

hek293t

Type

Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)

Abcam user community

Verified customer

提交于 Nov 27 2013

Application

Flow Cytometry

Sample

Human Cell (Human osteosarcoma U2OS cells)

Specification

Human osteosarcoma U2OS cells

Preparation

Cell harvesting/tissue preparation method: Trypsinization
Sample buffer: 1xPBS + 1 mg/ml BSA + 1 microg/ml DAPI + 250 microg/ml RNase

Fixation

Paraformaldehyde

Permeabilization

Yes - 1xPBS + 0.2% Triton X-100

Gating Strategy

FS vs. SS for debris, DAPI area vs. linear for doublets

Abcam user community

Verified customer

提交于 Aug 17 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Fixative

Formaldehyde

Permeabilization

Yes - 0.5% Triton X-100

Blocking step

BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Oct 28 2011

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (skin fibroblasts)

Specification

skin fibroblasts

Fixative

Formaldehyde

Permeabilization

Yes - 0.5% Triton X100

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Kok-Lung Chan

Verified customer

提交于 Nov 08 2007

1-10 of 11 Abreviews or Q&A

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Key features and details

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

骨髓瘤

同种型

轻链类型

研究领域

相关产品

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

应用

靶标

功能

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

文献 (137)

客户评价及客户问答

Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

ChIP abreview for Anti-RPA32/RPA2 antibody [9H8]

Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

Western blot abreview for Anti-RPA32/RPA2 antibody [9H8]

Western blot abreview for Anti-RPA32/RPA2 antibody [9H8]

Immunoprecipitation abreview for Anti-RPA32/RPA2 antibody [9H8]

ChIP abreview for Anti-RPA32/RPA2 antibody [9H8]

Flow Cytometry abreview for Anti-RPA32/RPA2 antibody [9H8]

Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]

Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [9H8]