Anti-RPA32/RPA2抗体[9H8] (ab2175)
Key features and details
- Mouse monoclonal [9H8] to RPA32/RPA2
- Suitable for: Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-RPA32/RPA2抗体[9H8]
参阅全部 RPA32/RPA2 一抗 -
描述
小鼠单克隆抗体[9H8] to RPA32/RPA2 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, IP, IHC-P, IHC-Fr, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Full length protein (Human).
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阳性对照
- WB: TE 671, SK N BE and HeLa whole cell lysates. IHC-P: Human tonsil tissue. Flow Cyt: HeLa cells.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
纯度
IgG fraction -
克隆
单克隆 -
克隆编号
9H8 -
骨髓瘤
unknown -
同种型
IgG1 -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
Our Abpromise guarantee covers the use of ab2175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 32 kDa. | |
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. This antibody may be diluted to a titer of 1/50-1/100 in an ABC method. We suggest an incubation period of 30 minutes at room temperature. | |
| ICC/IF | Use at an assay dependent concentration. Used at a dilution of 1/300 for 2 hrs on mouse MEF cells (see Abreview for further details). |
靶标
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功能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻译后修饰
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
细胞定位
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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数据库链接
- Entrez Gene: 6118 Human
- Omim: 179836 Human
- SwissProt: P15927 Human
- Unigene: 703070 Human
- Unigene: 79411 Human
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别名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
图片
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![Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.cn/ps/products/2/ab2175/Images/ab2175-5443-anti-rpa32-rpa2-antibody-9h8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an Abreview submitted by Dr Kok-Lung Chanab2175 at 1/200 staining human skin fibroblasts by ICC/IF. The cells were treated with 2mM hydroxyurea for 16 hours, formaldehyde fixed and permeabilized with 0.5% Triton X100. They were then incubated with the primary antibody for 1.5 hours at 37°C. A Cy3® conjugated sheep anti-mouse antibody was used as the secondary.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.cn/ps/products/2/ab2175/Images/ab2175-5440-anti-rpa32-rpa2-antibody-9h8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [9H8] (ab2175)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, staining RPA32/RPA2 with ab2175. Staining was detected using DAB. -
![Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.cn/ps/products/2/ab2175/Images/ab2175-5442-anti-rpa32-rpa2-antibody-9h8-western-blot.jpg)
Western blot - Anti-RPA32/RPA2 antibody [9H8] (ab2175)All lanes : Anti-RPA32/RPA2 antibody [9H8] (ab2175) at 5 µg/ml
Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 32 kDa
Observed band size: 32 kDa -
![Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.cn/ps/products/2/ab2175/Images/ab2175-5441-anti-rpa32-rpa2-antibody-9h8-flow-cytometry.jpg)
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)This image is courtesy of an anonymous AbreviewAnti-RPA/32/RPA2 antibody [9H8] (ab2175) is used to measure DNA Double-Stranded Breaks resection in U2OS cells +/- Camptotechin (CPT).
ab2175 is used at 1/200 dilution in 1xPBS + 0.2% Triton X-100 for 1 hour at 25°C.
Secondary antibody: anti-mouse Molecular Probes Alexa Fluor® 488 Conjugated at 1/200 dilution.
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![Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)](https://www.abcam.cn/ps/products/2/ab2175/Images/ab2175-5439-anti-rpa32-rpa2-antibody-9h8-flow-cytometry.jpg)
Flow Cytometry - Anti-RPA32/RPA2 antibody [9H8] (ab2175)Overlay histogram showing HeLa cells stained with ab2175 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2175, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
文献 (152)
ab2175 被引用在 152 文献中.
- Lochab S et al. Mycobacterium tuberculosis exploits host ATM kinase for survival advantage through SecA2 secretome. Elife 9:N/A (2020). PubMed: 32223892
- Mooser C et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nat Commun 11:123 (2020). PubMed: 31913317
- Zhang B et al. NBS1 is required for SPO11-linked DNA double-strand break repair in male meiosis. Cell Death Differ 27:2176-2190 (2020). PubMed: 31965061
- Thompson LL et al. Reduced SKP1 Expression Induces Chromosome Instability through Aberrant Cyclin E1 Protein Turnover. Cancers (Basel) 12:N/A (2020). PubMed: 32106628
- Pedersen H et al. Replication Protein A (RPA) Mediates Radio-Resistance of Glioblastoma Cancer Stem-Like Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32111042
