The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.1 - 1 µg/ml. Approx 85kDa band seen in Mouse Brain and Human Brain (Cerebellum) lysate [Predicted MW of approx. 80.8kDa according to NP_006449.2].
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Expressed in brain, heart, uterus and testis.
Belongs to the TRIM/RBCC family. Contains 1 B box-type zinc finger. Contains 1 filamin repeat. Contains 6 NHL repeats. Contains 1 RING-type zinc finger.
The interaction with MYO5B is dependent upon its NHL repeats, which form a beta-propeller (NHL) domain containing six blades.
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunoprecipitation - Anti-RNF22 antibody (ab4215)This image is courtesy of an Abreview submitted by Dr Christoph Maas
ab4215 immunoprecipitating RNF22 from rat brain tissue whole lysate. The antibody was used at a concentration of 2.5ug/mg of lysate. A Protein G matrix was used. The same antibody was used in the Western blot stage of the experiment (left hand panel).