概述

  • 产品名称
  • 描述
    兔多克隆抗体to RNF125
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human RNF125.

  • 阳性对照
    • Extracts from K562 and HT-29 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

靶标

  • 相关性
    RNF125 is a novel E3 ubiquitin ligase that is expressed in CD4 and CD8 positive T cells and contains an N-terminal RING finger domain. It may function as a positive regulator in the T-cell receptor signaling pathway. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase RNF125 antibody
    • Ring finger protein 125 antibody
    • RNF 125 antibody
    • T cell RING activation protein 1 antibody
    • T cell ring protein identified in activation screen antibody
    • TRAC 1 antibody
    see all

图片

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 100 kDa (possible non-specific binding)


    Exposure time: 1 minute

    See Abreview

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 100 kDa, 23 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Luo H  et al. Human bocavirus VP2 upregulates IFN-ß pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. J Immunol 191:660-9 (2013). Read more (PubMed: 23772026) »
See 1 Publication for this product

客户评价及客户问答

Answer

Thank you for taking the time to contact us. Your enquiries havebeen forwarded to the scientificsupport team.I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to reassure you that ab74373 is tested and covered by our 6 month guarantee for use inWB and onmouse and ratsamples. I am sorry to confirmthat because we carry over 80 000 products, it is not feasible for us to arrange sample vials. However, in the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement if it has been purchased within the guarantee period.

Reviewing this case, I would like to offer some suggestions and considerations to help optimize the results. I would also appreciate if you can confirm some further details:

1. Please confirm the order number and date of purchase.

2. There are inherent difficulties with antibody detection of recombinant tagged proteins that I can recommend considering. This may be a biological artifact rather than a problem with the antibody. Could you confirm that the protein transfected is full length and includes the immunogen sequence? Also, if the recombinant RNF125 is tagged, it is possible that the tag is preventing access to the antibody. Could you confirm at which region of the protein the tag has been placed?

3. Could you confirm the species from which the recombinantRNF125 gene was taken?

4. Could you confirm how the stability of transfection has been assessed?

5. I can recommend that due to the points described above, it is important toinclude an endogenous positive control sample. Could you provide details of the endogenous positive control that has been used? For example, this antibody has been used successfully on K562 and HT-29cells.

With regards to the conference, as far as I am aware we do not have any representatives attending this particular AIDS conference in Washington. One of my colleagues is currently checking this with our US office. If I have any more information, I will let you know as soon as possible.

We also have thefollowing new literature for AIDSrelated products thatmay beuseful to you:
https://docs.abcam.com/pdf/microbiology/HIV-antibodies-and-proteins.pdf

I hope this information is helpful, thank you for your cooperation. Should the suggestions notimprove the results, please do not hesitate to contact me again with the further requested details including results from the positive control.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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