The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
RNF125 is a novel E3 ubiquitin ligase that is expressed in CD4 and CD8 positive T cells and contains an N-terminal RING finger domain. It may function as a positive regulator in the T-cell receptor signaling pathway. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.
Luo H et al. Human bocavirus VP2 upregulates IFN-ß pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. J Immunol191:660-9 (2013).
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