概述

  • 产品名称
  • 描述
    兔多克隆抗体to RNF125
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    Synthetic peptide derived from an internal region of human RNF125.

  • 阳性对照
    • Extracts from K562 and HT-29 cells.

性能

应用

Our Abpromise guarantee covers the use of ab74373 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/10000.
WB 1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).

靶标

  • 相关性
    RNF125 is a novel E3 ubiquitin ligase that is expressed in CD4 and CD8 positive T cells and contains an N-terminal RING finger domain. It may function as a positive regulator in the T-cell receptor signaling pathway. E3 ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins.
  • 数据库链接
  • 别名
    • E3 ubiquitin protein ligase RNF125 antibody
    • Ring finger protein 125 antibody
    • RNF 125 antibody
    • T cell RING activation protein 1 antibody
    • T cell ring protein identified in activation screen antibody
    • TRAC 1 antibody
    see all

图片

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/200 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : Mouse ES (129Sv) whole cell lysate

    Lysates/proteins at 5 µg per lane.

    Secondary
    All lanes : HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 100 kDa (possible non-specific binding)


    Exposure time: 1 minute

    See Abreview

  • All lanes : Anti-RNF125 antibody (ab74373) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from HT-29 cells
    Lane 3 : Extracts from K562 cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 26 kDa
    Observed band size: 26 kDa
    Additional bands at: 100 kDa, 23 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

This product has been referenced in:
  • Luo H  et al. Human bocavirus VP2 upregulates IFN-ß pathway by inhibiting ring finger protein 125-mediated ubiquitination of retinoic acid-inducible gene-I. J Immunol 191:660-9 (2013). Read more (PubMed: 23772026) »

See 1 Publication for this product

客户评价及客户问答

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Application
Western blot
Loading amount
5 µg
Gel Running Conditions
Reduced Denaturing (10% gel)
Sample
Mouse Cell lysate - whole cell (mouse ES cells)
Specification
mouse ES cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Nov 11 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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