参阅全部 RLF 一抗
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Human
预测可用于: Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Macaque monkey, Gorilla, Chinese hamster, Orangutan
Synthetic peptide conjugated to KLH derived from within residues 1850 to the C-terminus of Human RLF.
- This antibody gave a positive signal in HeLa whole cell lysate, as well as wild-type Mouse embryo head tissue lysate.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab115011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 280 kDa (predicted molecular weight: 218 kDa).|
功能May be involved in transcriptional regulation.
组织特异性Widely expressed in fetal and adult tissues.
序列相似性Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 14 C2H2-type zinc fingers.
- Information by UniProt
- Rearranged L-myc fusion gene protein antibody
- RLF antibody
- RLF_HUMAN antibody
Anti-RLF antibody (ab115011) at 1/500 dilution + Wild-type Mouse Embryo Head tissue at 20 µg
Anti-Rabbit HRP Conjugated
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 218 kDa
Observed band size: 280 kDa why is the actual band size different from the predicted?
Additional bands at: 140 kDa (possible cross reactivity)
All lanes : Anti-RLF antibody (ab115011) at 1/500 dilution
All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
All lanes : Anti-Rabbit HRP Conjugated
Predicted band size: 218 kDa
Observed band size: 265 kDa why is the actual band size different from the predicted?
Additional bands at: 135 kDa (possible cross reactivity), 150 kDa (possible cross reactivity)
This product has been referenced in:
- Harten SK et al. The recently identified modifier of murine metastable epialleles, Rearranged L-Myc Fusion, is involved in maintaining epigenetic marks at CpG island shores and enhancers. BMC Biol 13:21 (2015). WB . Read more (PubMed: 25857663) »
- Daxinger L et al. An ENU mutagenesis screen identifies novel and known genes involved in epigenetic processes in the mouse. Genome Biol 14:R96 (2013). Read more (PubMed: 24025402) »