概述

  • 产品名称
  • 描述
    兔多克隆抗体to RIP3
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Rat RIP3 (C terminal).
    Database link: Q9Z2P5

  • 阳性对照
    • BALB/3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate IF/ICC: SV40LT-SMC cell line

性能

应用

Our Abpromise guarantee covers the use of ab62344 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Blocking peptide available ab8464

ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.

靶标

  • 功能
    Promotes apoptosis.
  • 组织特异性
    Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers.
  • 序列相似性
    Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • 翻译后修饰
    Autophosphorylated.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Receptor interacting protein 3 antibody
    • Receptor interacting serine threonine kinase 3 antibody
    • Receptor interacting serine/threonine protein kinase 3 antibody
    • Receptor-interacting protein 3 antibody
    • Receptor-interacting serine/threonine-protein kinase 3 antibody
    • RIP 3 antibody
    • RIP like protein kinase 3 antibody
    • RIP-3 antibody
    • RIP-like protein kinase 3 antibody
    • RIPK 3 antibody
    • RIPK3 antibody
    • RIPK3_HUMAN antibody
    see all

图片

  • All lanes : Anti-RIP3 antibody (ab62344) at 1 µg/ml

    Lane 1 : Whole cell lysate in the absence of RIP3 peptide
    Lane 2 : Whole cell lysate in the presence of RIP3 peptide

    Predicted band size: 57 kDa
    Observed band size: 57 kDa

  • ab62344 at 5 µg/ml staining rat kidney tissue by IHC-P.
  • Immunofluorescence of RIP3 in Rat Kidney cells using ab62344 at 20 ug/ml.

  • ICC/IF image of ab62344 stained SV40LT-SMC cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62344, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Wang W  et al. Tauroursodeoxycholic Acid Protects Nucleus Pulposus Cells from Compression-Induced Apoptosis and Necroptosis via Inhibiting Endoplasmic Reticulum Stress. Evid Based Complement Alternat Med 2018:6719460 (2018). Read more (PubMed: 29721028) »
  • Huang G  et al. Necroptosis in 3-chloro-1, 2-propanediol (3-MCPD)-dipalmitate-induced acute kidney injury in vivo and its repression by miR-223-3p. Toxicology 406-407:33-43 (2018). Read more (PubMed: 29860048) »

See all 7 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
BSA 1% + DK serum 5% as blocking agent for 2 hour(s) and 0 minute(s) · Temperature: 20°C
Fixative
Paraformaldehyde
Username

Mrs. Francoise Geffroy

Verified customer

提交于 Jan 24 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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