Anti-RIL抗体(ab6045)
Key features and details
- Goat polyclonal to RIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
重组抗体的批间可重复性更强
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-RIL抗体
参阅全部 RIL 一抗 -
描述
山羊多克隆抗体to RIL -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chicken, Cow -
免疫原
Synthetic peptide:
DVVAVYPNAKVELV
, corresponding to C terminal amino acids 317-330 of Human RIL. -
常规说明
GenBank Accession Number – NP_003678.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
l -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab6045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
---|---|---|
WB |
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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组织特异性
Isoform 2 is found in brain. -
序列相似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
翻译后修饰
Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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数据库链接
- Entrez Gene: 395643 Chicken
- Entrez Gene: 515410 Cow
- Entrez Gene: 8572 Human
- Entrez Gene: 30794 Mouse
- Omim: 603422 Human
- SwissProt: Q9PW72 Chicken
- SwissProt: Q3T005 Cow
- SwissProt: P50479 Human
see all -
别名
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
图片
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Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane).
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 1 hour
ab6045 at 0.2µ g/ml staining approximately 37kDa band of RIL in A431 Lysate by Western blot (ECL).
数据表及文件
文献 (3)
ab6045 被引用在 3 文献中.
- Miyazaki K et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res 318:1716-25 (2012). WB, ICC/IF ; Rat . PubMed: 22659164
- Guryanova OA et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem 286:26849-59 (2011). WB . PubMed: 21636573
- Iida Y et al. Localization of reversion-induced LIM protein (RIL) in the rat central nervous system. Acta Histochem Cytochem 42:9-14 (2009). WB, IHC-FoFr ; Rat . PubMed: 19293990
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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