Anti-RIL抗体(ab6045)

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Western blot - Anti-RIL antibody (ab6045)

    Key features and details

    • Goat polyclonal to RIL
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    重组抗体的批间可重复性更强

    Product image
    Anti-RIL antibody [EPR16199] (ab197026)
    • 研究可靠 —— 各批次间结果一致且可重复
    • 长期批量供应 —— 采用重组技术，可实现快速生产
    • 首次实验即可成功 —— 经过大量验证确认了特异性
    • 符合伦理标准 —— 产品不含动物成分

    概述

    • 产品名称

      Anti-RIL抗体
      参阅全部 RIL 一抗

    • 描述

      山羊多克隆抗体to RIL

    • 宿主

      Goat

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Chicken, Cow

    • 免疫原

      Synthetic peptide:

      DVVAVYPNAKVELV

      , corresponding to C terminal amino acids 317-330 of Human RIL.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      GenBank Accession Number – NP_003678.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    应用

    Our Abpromise guarantee covers the use of ab6045 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB
  • 应用说明
    IHC-FoFr: Use at an assay dependent dilution (PMID 19293990).
    WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • 靶标

    图片

    • Western blot - Anti-RIL antibody (ab6045)
      Western blot - Anti-RIL antibody (ab6045)
      Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane).

      Developed using the ECL technique.

      Predicted band size: 35 kDa


      Exposure time: 1 hour


      ab6045 at 0.2µg/ml staining approximately 37kDa band of RIL in A431 Lysate by Western blot (ECL).

    文献 (3)

    发表研究结果有使用 ab6045？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab6045 被引用在 3 文献中.

    • Miyazaki K  et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res 318:1716-25 (2012). WB, ICC/IF ; Rat . PubMed: 22659164
    • Guryanova OA  et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem 286:26849-59 (2011). WB . PubMed: 21636573
    • Iida Y  et al. Localization of reversion-induced LIM protein (RIL) in the rat central nervous system. Acta Histochem Cytochem 42:9-14 (2009). WB, IHC-FoFr ; Rat . PubMed: 19293990

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