Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Eye)
Antigen retrieval step
None
Permeabilization
Yes - Tween 0.1%
Specification
Eye
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2% BSA in PBS 0.1% tween
Dilution
1/10000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Donkey anti mouse AF 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Onkar Sawant
Verified customer
提交于 Jan 28 2016