Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Zebrafish Tissue sections (Long double cone (red cone) photoreceptor cells)
Specification
Long double cone (red cone) photoreceptor cells
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated anti-mouse antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000
Additional data
Additional Notes
Anti-Rhodopsin (1D4) antibody does not react with zebrafish rhodopsin, but most probably reacts with red opsin (LWS1, LWS2) in zebrafish as it stains outer segments of long double cone (red cone) photoreceptor cells in zebrafish.
PubMed ID
22743318: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Jun Yin
Verified customer
提交于 Jul 02 2013