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Signal Transduction Signaling Pathway G Protein Signaling GPCR

Anti-Rhodopsin抗体[1D4] (ab5417)

  • Datasheet
  • SDS
Reviews (2)Q&A (1)References (58)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

Key features and details

  • Mouse monoclonal [1D4] to Rhodopsin
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Rhodopsin抗体[1D4]
    参阅全部 Rhodopsin 一抗
  • 描述

    小鼠单克隆抗体[1D4] to Rhodopsin
  • 宿主

    Mouse
  • 特异性

    ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.

  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Zebrafish
  • 免疫原

    Other Immunogen Type corresponding to Bovine Rhodopsin.

  • 表位

    The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
  • 阳性对照

    • WB: HL60 whole cell lysate IHC-P: Human and mouse retinal tissue
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 纯度

    Protein G purified
  • Primary antibody说明

    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
  • 克隆

    单克隆
  • 克隆编号

    1D4
  • 同种型

    IgG1
  • 研究领域

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

应用

Our Abpromise guarantee covers the use of ab5417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/1000. Detects a band of approximately 40 kDa.
IHC-P 1/100 - 1/1000.

靶标

  • 功能

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 组织特异性

    Rod shaped photoreceptor cells which mediates vision in dim light.
  • 疾病相关

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 序列相似性

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻译后修饰

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 细胞定位

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Target information above from: UniProt accession P08100 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 100307115 Human
    • Entrez Gene: 6010 Human
    • Entrez Gene: 212541 Mouse
    • Entrez Gene: 30295 Zebrafish
    • Omim: 180380 Human
    • SwissProt: P08100 Human
    • SwissProt: P15409 Mouse
    • SwissProt: P35359 Zebrafish
    • Unigene: 247565 Human
    • Unigene: 2965 Mouse
    • Unigene: 406156 Mouse
    • Unigene: 354 Zebrafish
    see all
  • 别名

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)Pearring et al PLoS Genet. 2017 Apr 14;13(4):e1006740. doi: 10.1371/journal.pgen.1006740. eCollection 2017 Apr. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Deletion of Arf4 mice from the retina does not disrupt rhodopsin localization or photoreceptor morphology.

    E. Arf4 immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Image of the photoreceptor IS where the biosynthetic membranes are localized. Eyes were collected at P34. Scale bar = 10 μm.

    F. Rhodopsin immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P34. Scale bar = 10 μm.

    G. Comparative analysis of photoreceptor morphology in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P41. Scale bar = 20 μm.

    OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer.

  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 (Human promyelocytic leukemia cell line) cell lysate at 25 µg

    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed human retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded humanretinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Subsequent to retrival the retinal tissue was blocked in 3% H2O2-methanol for 15 min at room temperature. The sample was then incubated with ab5417 in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

实验方案

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (58)

    发表研究结果有使用 ab5417?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5417 被引用在 58 文献中.

    • Wu Y  et al. The molecular chaperone Hsp90a deficiency causes retinal degeneration by disrupting Golgi organization and vesicle transportation in photoreceptors. J Mol Cell Biol 12:216-229 (2020). PubMed: 31408169
    • Wright CB  et al. Chronic Dicer1 deficiency promotes atrophic and neovascular outer retinal pathologies in mice. Proc Natl Acad Sci U S A 117:2579-2587 (2020). PubMed: 31964819
    • Liu Y  et al. Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Sci Rep 10:7795 (2020). PubMed: 32385361
    • Yi Z  et al. Comparative exome sequencing reveals novel candidate genes for retinitis pigmentosa. EBioMedicine 56:102792 (2020). PubMed: 32454406
    • Deveau C  et al. Thyroid hormone receptor beta mutations alter photoreceptor development and function in Danio rerio (zebrafish). PLoS Genet 16:e1008869 (2020). PubMed: 32569302
    View all Publications for this product

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    Western blot abreview for Anti-Rhodopsin [1D4] antibody

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    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Sample
    Human Cell lysate - whole cell (neuroblast)
    Specification
    neuroblast
    Blocking step
    BSA as blocking agent for 50 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 17 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-Rhodopsin [1D4] antibody

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    Abreviews
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    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample
    Zebrafish Tissue sections (Long double cone (red cone) photoreceptor cells)
    Specification
    Long double cone (red cone) photoreceptor cells
    Permeabilization
    Yes - PBST
    Fixative
    Paraformaldehyde
    Read More

    Dr. Jun Yin

    Verified customer

    提交于 Jul 02 2013

    Question

    Would like to try this antibody in IP.

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    Abcam community

    Verified customer

    Asked on Jul 16 2012

    Answer

    Thank you very much for your call today. It was a pleasure speaking to you. I've created the discount code below, and if you have any further questions about redeeming the code or submitting the Abreview, please let me know and I'll be happy to help.

    DISCOUNT CODE: 10***
    Expiration date: November 17, 2012

    This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab5417 in IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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