Key features and details
- Rhodamine Goat polyclonal to Fluorescein
- Suitable for: Immunomicroscopy, Flow Cyt
- Reacts with: Species independent
- Conjugation: Rhodamine. Ex: 550nm, Em: 570nm
- Isotype: IgG
参阅全部 Fluorescein 一抗
偶联物Rhodamine. Ex: 550nm, Em: 570nm
经测试应用适用于: Immunomicroscopy, Flow Cytmore details
种属反应性与反应: Species independent
Chemical/ Small Molecule corresponding to Fluorescein. Fluorescein conjugated to Goat IgG
Tetramethylrhodamine isothiocyanante (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 1.6 moles TRITC per mole of Goat IgG.
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存放说明Shipped at 4°C. Store at +4°C.
存储溶液Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 1% BSA, 0.42% Potassium phosphate
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纯化说明This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
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相关性Fluorescein is a fluorophore commonly used to label proteins - protein-fluorescein conjugates are not usually susceptible to precipitation. In addition to its relatively high absorptivity, excellent fluorescence quantum yield and good water solubility, fluorescein has an excitation maximum of 494 nm that closely matches the 488 nm spectral line of the argon-ion laser, making it an important fluorophore for confocal laser-scanning microscopy and flow cytometry applications. Its fluorescence is pH sensitive and is significantly reduced below pH 7. Fluorescein emits most strongly between 500 and 550 nm, but it has a relatively broad emission spectrum reaching to over 600 nm. Several derivatives of fluorescein are commonly used, including FITC (fluorescein isothiocyanate), carboxylates and succinimidyl esters.
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