重组Anti-RhD抗体[D7C2] (ab281912)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [D7C2] to RhD
- Suitable for: ICC/IF
- Reacts with: Human
概述
-
产品名称
Anti-RhD抗体[D7C2]
参阅全部 RhD 一抗 -
描述
人单克隆抗体[D7C2] to RhD -
宿主
Human -
经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Tissue, cells or virus corresponding to Human RhD. Rh- volunteer blood donor (r/r) was injected with 5ml of Rh+ erythrocytes (R1/r). Isolated peripheral lymphocytes were transformed with 1 ml of culture supernatant of the EBV marmoset B96-8 cell line.
Database link: Q02161 -
阳性对照
- ICC/IF: K562 cells.
-
常规说明
ab281912 is the recombinant human IgM version of ab281911.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading...
-
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
D7C2 -
同种型
IgM -
轻链类型
lambda -
研究领域
相关产品
-
Alternative Versions
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab281912于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
ICC/IF |
Use a concentration of 10 µg/ml.
|
说明 |
---|
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
-
功能
May be part of an oligomeric complex which is likely to have a transport or channel function in the erythrocyte membrane. -
组织特异性
Restricted to tissues or cell lines expressing erythroid characters. -
序列相似性
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
细胞定位
Membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 6007 Human
- Omim: 111680 Human
- SwissProt: Q02161 Human
- Unigene: 449968 Human
-
别名
- Blood group Rh(D) polypeptide antibody
- Blood group--Rhesus system D polypeptide antibody
- CD240D antibody
see all
图片
-
This data was developed using the chimeric version (ab281911).
Immunofluorescence analysis of paraformaldehyde fixed K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) cells labelling RhD with ab281911 at 10 µg/ml for 1hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. K562 cells were immobilized on Shi-fix™ coverslips.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab281912 尚未被引用在任何文献中。