Anti-RGS3抗体(ab2564)
Key features and details
- Rabbit polyclonal to RGS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RGS3抗体
参阅全部 RGS3 一抗 -
描述
兔多克隆抗体to RGS3 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).
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常规说明
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
Primary antibody说明
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa). |
靶标
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功能
Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. -
序列相似性
Contains 1 C2 domain.
Contains 1 PDZ (DHR) domain.
Contains 1 RGS domain. -
翻译后修饰
Phosphorylated by cyclic GMP-dependent protein kinase.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear. - Information by UniProt
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数据库链接
- Entrez Gene: 5998 Human
- Omim: 602189 Human
- SwissProt: P49796 Human
- Unigene: 494875 Human
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别名
- C2PA antibody
- FLJ20370 antibody
- FLJ31516 antibody
see all
图片
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Western blot - Anti-RGS3 antibody (ab2564)Lane 1 : Anti-RGS3 antibody (ab2564)
Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml
Lane 1 : mock
Lane 2 : transfected HEK293 cells (right lane)
Predicted band size: 132 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
数据表及文件
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Datasheet download
文献 (0)
ab2564 尚未被引用在任何文献中。
