Anti-RGS3抗体(ab2564)
Key features and details
- Rabbit polyclonal to RGS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RGS3抗体
参阅全部 RGS3 一抗 -
描述
兔多克隆抗体to RGS3 -
宿主
Rabbit -
Tested Applications & Species
Application Species WB Human -
免疫原
E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).
-
常规说明
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading...
-
纯度
Immunogen affinity purified -
Primary antibody说明
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
---|---|
WB |
Human
|
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa).
|
说明 |
---|
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa). |
靶标
-
功能
Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. -
序列相似性
Contains 1 C2 domain.
Contains 1 PDZ (DHR) domain.
Contains 1 RGS domain. -
翻译后修饰
Phosphorylated by cyclic GMP-dependent protein kinase.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear. - Information by UniProt
-
数据库链接
- Entrez Gene: 5998 Human
- Omim: 602189 Human
- SwissProt: P49796 Human
- Unigene: 494875 Human
-
别名
- C2PA antibody
- FLJ20370 antibody
- FLJ31516 antibody
see all
图片
-
Lane 1 : Anti-RGS3 antibody (ab2564)
Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml
Lane 1 : mock
Lane 2 : transfected HEK293 cells (right lane)
Predicted band size: 132 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab2564 尚未被引用在任何文献中。