概述

  • 产品名称
  • 描述
    鸡多克隆抗体to RGS12
  • 宿主
    Chicken
  • 经测试应用
    适用于: WB, ICCmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GSGTHGSRD LPVNRIIDVD LVTGSAPGRD GGIAGAQAGP GRSQASGGPP TSDLPGLGPV PGEPAKPKTS AHHATFV

    , corresponding to amino acids 1372-1447 of RGS12.

  • 阳性对照
    • E coli-derived fusion protein.
  • 常规说明
    During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

性能

相关产品

应用

Our Abpromise guarantee covers the use of ab14258 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Predicted molecular weight: 174 kDa.
ICC Use at an assay dependent concentration.

靶标

  • 相关性
    RGS12 (Regulator of G protein signalling 12) may function as a guanosine triphosphatase (GTPase)-activating protein as well as a transcriptional repressor. This protein may play a role in tumorigenesis. Multiple transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their biological nature has not been determined.
  • 细胞定位
    Nuclear
  • 数据库链接
  • 别名
    • DKFZp761K1617 antibody
    • Regulator of G protein signalling 12 antibody

图片

  • WB using E coli-derived fusion protein as test antigen. Affi-anti- RGS12b IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development. WB using E coli-derived fusion protein as test antigen. Affi-anti- RGS12b IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.
  • ab14258 staining RGS12 in cultured Human glioblastoma cells by Immunocytochemistry. Cells were PFA-fixed and permeabilized in 0.1% Triton X-100 in PBS prior to blocking in 0.5% BSA in TBS-Tween for 20 minutes at room temperature. The primary antibody was diluted 1/50 in 0.5% BSA/PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Biotin-conjugated Goat anti-Chicken polyclonal, diluted 1/400.

    See Abreview

文献

ab14258 has not yet been referenced specifically in any publications.

客户评价及客户问答

1-2 of 2 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Human Cultured Cells (human glioblastoma cell lines)
Specification
human glioblastoma cell lines
Fixative
Paraformaldehyde
Permeabilization
Yes - Tritonx100 0.1% in PBS
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Feb 15 2011

Answer

I have some additional information for you. Yes, the antigen was a fusion protein containing a partial sequence and that is why the protein runs at a smaller size. We have not had other complaints about this antibody. We don't have a specific protocol for this antibody, but we do have a general one recommended for Western Blot performed with IgY. Method 1. Separate appropriate amount of cell lysates by 10-20% SDS-PAGE, followed by transferring to PVDF membrane. 2. Block membrane with 5% non-fat milk in TBS-T (Tris-buffered saline containing 0.05% Tween, pH 7.4) for 1 hour at room temperature or longer at 4C. (BSA is not recommended as a blocking reagent). 3. Rinse membrane with TBS-T. 4. Incubate membrane with IgY antibodies appropriately diluted with 5% milk in TBS-T @ R.T. for 1 h. 5. Wash membrane with TBS-T, 3 min each, total of 3 times. 6. Incubate with 2nd antibody (goat-anti-IgY/Fc-HRP) at dilution 1:1,000 for colorimetric assay or 1:10,000 - 1:100,000 for ECL (with 0.5% milk TBS-T) @ R.T. for 1 h. 7. Wash with TBS-T, 3-5 min each with shaking, total of 3 times. 8. Perform color development. Or perform ECL detection of signal using ECL kit. I hope this information helps, please do not hesitate to contact us if you need any more advice or information,

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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