Anti-RFC3抗体(ab154899)
Key features and details
- Rabbit polyclonal to RFC3
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFC3抗体
参阅全部 RFC3 一抗 -
描述
兔多克隆抗体to RFC3 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 51-353 of Human RFC3 (Uniprot ID: P40938).
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阳性对照
- HeLa whole cell lysate; HeLa cells; Human gastric carcinoma tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab154899于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 41 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 41 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. -
序列相似性
Belongs to the activator 1 small subunits family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5983 Human
- Omim: 600405 Human
- SwissProt: P40938 Human
- Unigene: 115474 Human
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别名
- A1 38 kDa subunit antibody
- Activator 1 38 kDa subunit antibody
- Activator 1 subunit 3 antibody
see all
图片
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Anti-RFC3 antibody (ab154899) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 41 kDa
10% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling RFC3 with ab154899 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human gastric carcinoma tissue labeling RFC3 with ab154899 at 1/500 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab154899 被引用在 4 文献中.
- Hoffmann S et al. FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease. EMBO Rep 21:e50662 (2020). PubMed: 32776417
- Panda D et al. Triad of human cellular proteins, IRF2, FAM111A, and RFC3, restrict replication of orthopoxvirus SPI-1 host-range mutants. Proc Natl Acad Sci U S A 114:3720-3725 (2017). PubMed: 28320935
- He ZY et al. Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT. Transl Oncol 10:1-9 (2017). WB, IHC-P ; Human . PubMed: 27888707
- Shen H et al. ShRNA-mediated silencing of the RFC3 gene suppress ovarian tumor cells proliferation. Int J Clin Exp Pathol 8:8968-75 (2015). PubMed: 26464638