Anti-RFC1抗体(ab3853)
Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
-
产品名称
Anti-RFC1抗体
参阅全部 RFC1 一抗 -
描述
兔多克隆抗体to RFC1 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Gorilla
-
免疫原
Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.
-
阳性对照
- Nuclear extract from HeLa cells.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP |
Use at 2 µg/mg of lysate.
|
| 说明 |
|---|
|
IP
Use at 2 µg/mg of lysate. |
靶标
-
功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
组织特异性
Wide tissue distribution. Undetectable in placental tissue. -
序列相似性
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 5981 Human
- Entrez Gene: 19687 Mouse
- Omim: 102579 Human
- SwissProt: P35251 Human
- SwissProt: P35601 Mouse
- Unigene: 507475 Human
- Unigene: 148877 Mouse
-
别名
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
图片
-
Immunoprecipitation - Anti-RFC1 antibody (ab3853)
Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)
Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.
Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).
WB - 0.5
µ g/ml, IP - 2µ g/mg NE.Detection: ECL, 30 seconds
数据表及文件
-
SDS download
-
Datasheet download
文献 (4)
ab3853 被引用在 4 文献中.
- Wessel SR et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
- Castaño E et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
- Lee KY et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
- Lee KY et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293