Anti-RFC1抗体(ab3853)
Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFC1抗体
参阅全部 RFC1 一抗 -
描述
兔多克隆抗体to RFC1 -
宿主
Rabbit -
经测试应用
适用于: IP, WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Gorilla
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免疫原
Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.
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阳性对照
- Nuclear extract from HeLa cells.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2 µg/mg of lysate. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 128 kDa). |
靶标
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功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
组织特异性
Wide tissue distribution. Undetectable in placental tissue. -
序列相似性
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5981 Human
- Entrez Gene: 19687 Mouse
- Omim: 102579 Human
- SwissProt: P35251 Human
- SwissProt: P35601 Mouse
- Unigene: 507475 Human
- Unigene: 148877 Mouse
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别名
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
图片
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Immunoprecipitation - Anti-RFC1 antibody (ab3853)Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)
Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.
Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).
WB - 0.5
µ g/ml, IP - 2µ g/mg NE.Detection: ECL, 30 seconds
文献 (4)
ab3853 被引用在 4 文献中.
- Wessel SR et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
- Castaño E et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
- Lee KY et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
- Lee KY et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293
