Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
参阅全部 RFC1 一抗
经测试应用适用于: WBmore details
预测可用于: Mouse, Chimpanzee, Gorilla
Synthetic peptide within Human RFC1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P35251
- WB: HeLa and HEK-293T whole cell lysates.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
纯度Immunogen affinity purified
功能The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.
组织特异性Wide tissue distribution. Undetectable in placental tissue.
序列相似性Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain.
翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
All lanes : Anti-RFC1 antibody (ab3853) at 0.4 µg/ml
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Predicted band size: 128 kDa
Exposure time: 3 minutes
ab3853 被引用在 4 文献中.
- Wessel SR et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
- Castaño E et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
- Lee KY et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
- Lee KY et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293