Anti-RFC1抗体(ab3853)

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Western blot - Anti-RFC1 antibody (ab3853)

    Key features and details

    • Rabbit polyclonal to RFC1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-RFC1抗体
      参阅全部 RFC1 一抗

    • 描述

      兔多克隆抗体to RFC1

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse, Chimpanzee, Gorilla

    • 免疫原

      Synthetic peptide within Human RFC1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
      Database link: P35251

    • 阳性对照

      • WB: HeLa and HEK-293T whole cell lysates.

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    • 存储溶液

      pH: 7
      Preservative: 0.1% Sodium azide
      Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3853于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1000 - 1/10000. Predicted molecular weight: 128 kDa.
    说明
    WB
    1/1000 - 1/10000. Predicted molecular weight: 128 kDa.

    靶标

    • 功能

      The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
      Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair.

    • 组织特异性

      Wide tissue distribution. Undetectable in placental tissue.

    • 序列相似性

      Belongs to the activator 1 large subunit family.
      Contains 1 BRCT domain.

    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • A1 140 kDa subunit antibody
        • A1 antibody
        • A1 P145 Activator 1 large subunit antibody
        • Activator 1 140 kDa subunit antibody
        • Activator 1 large subunit antibody
        • Activator 1 subunit 1 antibody
        • DNA binding Protein PO GA antibody
        • DNA-binding protein PO-GA antibody
        • MHC binding factor beta antibody
        • MHCBFB antibody
        • PO GA antibody
        • RECC1 antibody
        • Replication factor C (activator 1) 1, 145kDa antibody
        • Replication factor C 140 kDa subunit antibody
        • Replication factor C antibody
        • Replication factor C large subunit antibody
        • Replication factor C subunit 1 antibody
        • Replication factor C1 antibody
        • RF-C 140 kDa subunit antibody
        • RFC antibody
        • RFC1 antibody
        • RFC1_HUMAN antibody
        • RFC140 antibody
        • RFC140 Replication Factor C 140 kDa subunit antibody
        see all

      图片

      • Western blot - Anti-RFC1 antibody (ab3853)
        Western blot - Anti-RFC1 antibody (ab3853)
        All lanes : Anti-RFC1 antibody (ab3853) at 0.4 µg/ml

        Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg
        Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg
        Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

        Predicted band size: 128 kDa


        Exposure time: 3 minutes

      文献 (4)

      发表研究结果有使用 ab3853？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3853 被引用在 4 文献中.

      • Wessel SR  et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
      • Castaño E  et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
      • Lee KY  et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
      • Lee KY  et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293

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