Anti-RFC1抗体(ab3853)
Key features and details
- Rabbit polyclonal to RFC1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-RFC1抗体
参阅全部 RFC1 一抗 -
描述
兔多克隆抗体to RFC1 -
宿主
Rabbit -
经测试应用
适用于: IPmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Chimpanzee, Gorilla
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免疫原
Synthetic peptide (Human) - which represents a portion of human Replication Factor C 1, 140 kDa subunit encoded in part by exons 25 and 26.
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阳性对照
- Nuclear extract from HeLa cells.
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab3853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IP | Use at 2 µg/mg of lysate. |
靶标
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功能
The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins PCNA and activator 1. This subunit binds to the primer-template junction. Binds the PO-B transcription element as well as other GA rich DNA sequences. Could play a role in DNA transcription regulation as well as DNA replication and/or repair. Can bind single- or double-stranded DNA.
Interacts with C-terminus of PCNA. 5' phosphate residue is required for binding of the N-terminal DNA-binding domain to duplex DNA, suggesting a role in recognition of non-primer template DNA structures during replication and/or repair. -
组织特异性
Wide tissue distribution. Undetectable in placental tissue. -
序列相似性
Belongs to the activator 1 large subunit family.
Contains 1 BRCT domain. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 5981 Human
- Entrez Gene: 19687 Mouse
- Omim: 102579 Human
- SwissProt: P35251 Human
- SwissProt: P35601 Mouse
- Unigene: 507475 Human
- Unigene: 148877 Mouse
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别名
- A1 140 kDa subunit antibody
- A1 antibody
- A1 P145 Activator 1 large subunit antibody
see all
图片
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Immunoprecipitation - Anti-RFC1 antibody (ab3853)
Detection of Human RFC 1 by Western Blot and Immunoprecipitation using Affinity Purified Rabbit anti-RFC 1 (ab3853)
Sample: Nuclear Extract (NE, amounts used are indicated) from HeLa cells.
Antibody: Affinity Purified Rb anti-RFC 1 (ab3853).
WB - 0.5
µ g/ml, IP - 2µ g/mg NE.Detection: ECL, 30 seconds
文献 (4)
ab3853 被引用在 4 文献中.
- Wessel SR et al. Functional Analysis of the Replication Fork Proteome Identifies BET Proteins as PCNA Regulators. Cell Rep 28:3497-3509.e4 (2019). PubMed: 31553917
- Castaño E et al. Folate Transporters in Placentas from Preterm Newborns and Their Relation to Cord Blood Folate and Vitamin B12 Levels. PLoS One 12:e0170389 (2017). WB . PubMed: 28103309
- Lee KY et al. ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin. J Cell Biol 200:31-44 (2013). WB ; Human . PubMed: 23277426
- Lee KY et al. Human ELG1 regulates the level of ubiquitinated proliferating cell nuclear antigen (PCNA) through Its interactions with PCNA and USP1. J Biol Chem 285:10362-9 (2010). PubMed: 20147293
