Application
Western blot
Sample
Human Cell lysate - whole cell (Ovarian granulosa)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel)
Loading amount
500000 cells
Specification
Ovarian granulosa
Blocking step
Odyssey Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: RT°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking buffer + tween
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Licor
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680RD
Detection
Detection method
Infrared Flourescence
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 51 kDa
Additional data
Additional Notes
Lane 1 = KGN (granulosa cell line)
Lane 2 = GLC (primary granulosa-luteal cells)
Anti-RXR antibody (red band) detects a single band at approximately 51kDa.
Anti-betaActin antibody (loading control) detects a single band (green band) at approximately 45kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Suman Rice
Verified customer
提交于 Oct 19 2015