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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Retinoic Acid Receptor beta抗体(ab5792)

  • Datasheet
Reviews (1)Q&A (1)

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Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
  • Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab5792)

Key features and details

  • Rabbit polyclonal to Retinoic Acid Receptor beta
  • Suitable for: ICC/IF, WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

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Recombinant Human Retinoic Acid Receptor beta protein (ab82202)

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概述

  • 产品名称

    Anti-Retinoic Acid Receptor beta抗体
    参阅全部 Retinoic Acid Receptor beta 一抗
  • 描述

    兔多克隆抗体to Retinoic Acid Receptor beta
  • 宿主

    Rabbit
  • 特异性

    This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma.
  • 经测试应用

    适用于: ICC/IF, WBmore details
  • 种属反应性

    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Mouse Retinoic Acid Receptor beta aa 429-448.
    Sequence:

    PSUSPSSVENSGVSQSPLLQ


    (Peptide available as ab5897)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • WB: SH-SY5Y cell extract ; HEK-293 ICC/IF: SH-SY5Y
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • 纯度

    Whole antiserum
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Retinoic & Retinoid
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Retinoic & Retinoid
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Retanoic and retanoid

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Retinoic Acid Receptor beta protein (ab82202)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5792于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).
说明
ICC/IF
1/100.
WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).

靶标

  • 功能

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
  • 疾病相关

    Microphthalmia, syndromic, 12
  • 序列相似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 结构域

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
  • 细胞定位

    Cytoplasm and Nucleus.
  • Target information above from: UniProt accession P10826 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 5915 Human
    • Entrez Gene: 218772 Mouse
    • Omim: 180220 Human
    • SwissProt: P10826 Human
    • SwissProt: P22605 Mouse
    • Unigene: 543218 Human
    • Unigene: 581530 Human
    • Unigene: 654490 Human
    • Unigene: 733004 Human
    • Unigene: 259318 Mouse
    see all
  • 别名

    • HAP antibody
    • HBV-activated protein antibody
    • NR1B2 antibody
    • Nuclear receptor subfamily 1 group B member 2 antibody
    • RAR B antibody
    • RAR beta antibody
    • RAR epsilon antibody
    • RAR-beta antibody
    • RAR-epsilon antibody
    • RARB antibody
    • RARB_HUMAN antibody
    • Retinoic acid receptor beta 2 antibody
    • Retinoic acid receptor beta 4 antibody
    • Retinoic acid receptor beta 5 antibody
    • Retinoic acid receptor beta antibody
    • Retinoic acid receptor beta polypeptide antibody
    • RRB2 antibody
    see all

图片

  • Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
    Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
    All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1 µg/ml

    Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : PANC-1 (Human pancreatic epithelial cancinoma cell line) whole cell lysate
    Lane 4 : OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate
    Lane 5 : BeWo (human placenta choriocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Predicted band size: 53 kDa
    Additional bands at: ~58.50 kDa. We are unsure as to the identity of these extra bands.



    Detection: chemiluminescence 

    Western blot demonstrating antibody specificity by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SH-SY5Y and HEK-293 and not seen in PANC-1, OVCAR-3 and BeWo using ab5792.

  • Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
    Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
    All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1/1000 dilution

    Lane 1 : SH-SY5Y cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Mouse heart cell lysate

    Lysates/proteins at 25 µg per lane.

    Predicted band size: 53 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab5792)
    Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab5792)

    Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate cells on 70% confluent log phase labeling Retinoic Acid Receptor beta. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ab5792 at 1/100 dilution in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e represents BeWo (human placenta choriocarcinoma cell line) having no expression of Retinoic Acid Receptor beta. The images were captured at 60X magnification.

实验方案

  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab5792?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab5792 尚未被引用在任何文献中。

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-Retinoic Acid Receptor beta antibody

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Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Specification
brain
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 100x
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
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Abcam user community

Verified customer

提交于 Mar 29 2012

Question

send testing discount for ab5792 in IHC

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Abcam community

Verified customer

Asked on Feb 13 2012

Answer

Code de remise :xx Date d’expiration : 14. Juin 2012

Je suis ravie que vous souhaitiez participer a notre offre et tester ab5792 en IHC. Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire.

Vous devez dans un premier temps soumettre une Abreview avec vos résultats en IHC en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab5792.

Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

Termes et Conditions : www.abcam.com/collaborationdiscount.

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Abcam Scientific Support

回复于 Feb 13 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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