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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Retinoic Acid Receptor beta抗体(ab5792)

  • Datasheet
Reviews (1)Q&A (1)

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Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)

    Key features and details

    • Rabbit polyclonal to Retinoic Acid Receptor beta
    • Suitable for: WB
    • Reacts with: Mouse, Human, Saccharomyces cerevisiae
    • Isotype: IgG

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    概述

    • 产品名称

      Anti-Retinoic Acid Receptor beta抗体
      参阅全部 Retinoic Acid Receptor beta 一抗
    • 描述

      兔多克隆抗体to Retinoic Acid Receptor beta
    • 宿主

      Rabbit
    • 特异性

      This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma.
    • 经测试应用

      适用于: WBmore details
    • 种属反应性

      与反应: Mouse, Human, Saccharomyces cerevisiae
    • 免疫原

      Synthetic peptide corresponding to Mouse Retinoic Acid Receptor beta aa 429-448.
      Sequence:

      PSUSPSSVENSGVSQSPLLQ


      (Peptide available as ab5897)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 阳性对照

      • SH-SY5Y cell extract.
    • 常规说明

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    性能

    • 形式

      Liquid
    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • 存储溶液

      Preservative: 0.05% Sodium azide
      Constituent: 99% PBS
    • Concentration information loading...
    • 纯度

      Whole antiserum
    • 克隆

      多克隆
    • 同种型

      IgG
    • 研究领域

      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Zinc Finger
      • Signal Transduction
      • Signaling Pathway
      • Nuclear Signaling
      • Nuclear Hormone Receptors
      • Retinoic & Retinoid
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • Nuclear Receptors
      • Retinoic & Retinoid
      • Cancer
      • Signal transduction
      • Nuclear signaling
      • Nuclear hormone receptors
      • Retanoic and retanoid

    相关产品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Retinoic Acid Receptor beta protein (ab82202)

    应用

    Our Abpromise guarantee covers the use of ab5792 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB 1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).

    靶标

    • 功能

      Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function.
    • 疾病相关

      Microphthalmia, syndromic, 12
    • 序列相似性

      Belongs to the nuclear hormone receptor family. NR1 subfamily.
      Contains 1 nuclear receptor DNA-binding domain.
    • 结构域

      Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.
    • 细胞定位

      Cytoplasm and Nucleus.
    • Target information above from: UniProt accession P10826 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 数据库链接

      • Entrez Gene: 5915 Human
      • Entrez Gene: 218772 Mouse
      • Omim: 180220 Human
      • SwissProt: P10826 Human
      • SwissProt: P22605 Mouse
      • Unigene: 543218 Human
      • Unigene: 581530 Human
      • Unigene: 654490 Human
      • Unigene: 733004 Human
      • Unigene: 259318 Mouse
      see all
    • 别名

      • HAP antibody
      • HBV-activated protein antibody
      • NR1B2 antibody
      • Nuclear receptor subfamily 1 group B member 2 antibody
      • RAR B antibody
      • RAR beta antibody
      • RAR epsilon antibody
      • RAR-beta antibody
      • RAR-epsilon antibody
      • RARB antibody
      • RARB_HUMAN antibody
      • Retinoic acid receptor beta 2 antibody
      • Retinoic acid receptor beta 4 antibody
      • Retinoic acid receptor beta 5 antibody
      • Retinoic acid receptor beta antibody
      • Retinoic acid receptor beta polypeptide antibody
      • RRB2 antibody
      see all

    图片

    • Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
      Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)
      All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1/1000 dilution

      Lane 1 : SH-SY5Y cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : Mouse heart cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 53 kDa
      Observed band size: 58 kDa
      why is the actual band size different from the predicted?

    实验方案

    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • Datasheet
  • 文献 (0)

    发表研究结果有使用 ab5792?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5792 尚未被引用在任何文献中。

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Retinoic Acid Receptor beta antibody

    Poor
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Triton 100x
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 29 2012

    Question

    send testing discount for ab5792 in IHC

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2012

    Answer

    Code de remise :xx Date d’expiration : 14. Juin 2012

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab5792 en IHC. Le code de réduction correspondant est xx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre anticorps primaire.

    Vous devez dans un premier temps soumettre une Abreview avec vos résultats en IHC en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab5792.

    Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Feb 13 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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