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Resazurin Assay Kit (Cell Viability) ab129732 is a fluorometric/colorimetric assay that allows determination of the metabolic capacity of live cells in high throughput format.
The Resazurin assay uses an indicator dye to measure oxidation-reduction reactions which principally occur in the mitochondria of live cells. When reduced by metabolically active cells the non-fluorescent dark blue dye becomes fluorescent pink with absorbance at 570nm and red-fluorescent properties (560±10Ex/590Em) at neutral pH.
The resazurin dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity.
This kit was previously called Mitochondrial Viability Stain.
|ab129732 - 20x Mitochondrial viability stain||1 x 24ml|
Jurkat cells were seeded at 25,000 cells per well in a 50 µL volume and immediately overlayed with a 2X concentration of the indicated drug for a final volume of 100 µL. Cells were incubated for 2 hours with the indicated drug prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for the indicated drug was found at 22 – 25 µM.
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