Resazurin Assay试剂盒(Cell Viability) (ab129732)

概述

  • 产品名称
    Resazurin Assay试剂盒(Cell Viability)
  • 检测方法
    Fluorescent
  • 样品类型
    Adherent cells, Suspension cells
  • 检测类型
    Cell-based (quantitative)
  • 产品概述

    Resazurin Assay Kit (Cell Viability) ab129732 is a fluorometric/colorimetric assay that allows determination of the metabolic capacity of live cells in high throughput format.


    The Resazurin assay uses an indicator dye to measure oxidation-reduction reactions which principally occur in the mitochondria of live cells. When reduced by metabolically active cells the non-fluorescent dark blue dye becomes fluorescent pink with absorbance at 570nm and red-fluorescent properties (560±10Ex/590Em) at neutral pH.


    The resazurin dye can be measured in fluorescence or absorbance mode. However fluorescence mode measurement offers greater assay linearity, reproducibility, robustness and sensitivity.


    This kit was previously called Mitochondrial Viability Stain.


    Review our cell health assay guide to learn about our kits to perform a cell viability assay, cytotoxicity assay or cell proliferation assay.

  • 平台
    Reagents

性能

图片

  • Jurkat cells were seeded at 25,000 cells per well in a 50 µL volume and immediately overlayed with a 2X concentration of Staurosporin for a final volume of 100 µL. Cells were incubated for 4 hours with Staurosporin prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for staurosporin was found at 500 nM.
  • Jurkat cells were seeded at 25,000 cells per well in a 50 µL volume and immediately overlayed with a 2X concentration of the indicated drug for a final volume of 100 µL. Cells were incubated for 2 hours with the indicated drug prior to the addition of 2X stain diluted in RPMI media. After 4 hours of further incubation with stain, fluorescence was measured. IC50 for the indicated drug was found at 22 – 25 µM.

  • Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Colorimetric readout gave on average a Z factor of 0.72 on HepG2 cells seeded from 40,000 – 150,000 cells per well.
  • Dynamic range and suitability of the mitochondrial viability assay for high throughput screening. Cells were seeded in a titration series with n=11 for each data point. Fluorometric readout gave on average a Z factor of 0.82 on Jurkat cells seeded from 1,500 – 1000,000 cells per well on a 96-well plate.
  • Mitochondrial viability stain in long term toxicity. HepG2 cells previously cultured in glucose or galactose substrate media were seeded at 10,000 cells per well and allowed to adhere overnight. Media was replaced for the specific culture media in the presence of a titration series of Rotenone (5 µM – 0.5 pM). Cells were treated for 72 hours prior to the addition of 2X stain. After 4 hours of incubation plates were analyzed by fluorescent readout.
  • Effect of stain incubation time on assay reproducibility and background. Dark wall plates were seeded with a titration series of each cell line in a final volume of 100 µL of media. 2X stain (diluted in the cell line specific growth media) was added to each well at specified time points. XY graphs data is shown after media and background substraction. Bar graph shows mean background staining at different time points. 4 hour incubation of stain results in optimal reproducibility with minimal increase of background over the 2 hour incubation.

实验方案

文献

This product has been referenced in:
  • Deng F  et al. Inhibition of Caveolae Contributes to Propofol Preconditioning-Suppressed Microvesicles Release and Cell Injury by Hypoxia-Reoxygenation. Oxid Med Cell Longev 2017:3542149 (2017). Read more (PubMed: 29181124) »

See 1 Publication for this product

客户评价及客户问答

The colorimetric assay may be run on cell culture treated clear plastic plates.

Thank you for contacting us.


We only tested the cell lines shown in the graph, but the stain should work with any cell line (of any species).

I hope this information is helpful to you. Please do not hesitate to contact us if you ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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