Renin Assay试剂盒(Fluorometric) (ab138875)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Cell Lysate
- Sensitivity: 10 ng/ml
概述
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产品名称
Renin Assay试剂盒(Fluorometric)
参阅全部 Renin 试剂盒 -
检测方法
Fluorescent -
样品类型
Cell Lysate -
检测类型
Quantitative -
灵敏度
10 ng/ml -
种属反应性
与反应: Mammals, Other species -
产品概述
Renin Assay Kit (Fluorometric) (ab138875) provides a convenient assay for high throughput screening of renin inhibitors and for continuous assay of renin activity using a TF3/TQ3 FRET peptide. In the FRET peptide the fluorescence of TF3 is quenched by TQ3. Upon cleavage into two separate fragments by renin, the fluorescence of TF3 is recovered, the fluorescent signal can be easily monitored by a fluorescence microplate reader at Ex/Em = 540/590 nm. This assay is about fifty fold more sensitive than an EDANS/DABCYL-based assay.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer 1 x 10ml Renin Red Substrate (100x) 1 x 50µl Renin Standard 1 x 25µl -
研究领域
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功能
Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. -
疾病相关
Defects in REN are a cause of renal tubular dysgenesis (RTD) [MIM:267430]. RTD is an autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype).
Defects in REN are the cause of familial juvenile hyperuricemic nephropathy type 2 (HNFJ2) [MIM:613092]. It is a renal disease characterized by juvenile onset of hyperuricemia, slowly progressive renal failure and anemia. -
序列相似性
Belongs to the peptidase A1 family. -
细胞定位
Secreted. Membrane. Associated to membranes via binding to ATP6AP2. - Information by UniProt
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别名
- Angiotensin forming enzyme
- Angiotensin forming enzyme precursor
- Angiotensinogenase
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab138875 被引用在 4 文献中.
- Kang JO et al. A cardiac-null mutation of Prdm16 causes hypotension in mice with cardiac hypertrophy via increased nitric oxide synthase 1. PLoS One 17:e0267938 (2022). PubMed: 35862303
- Cansby E et al. Depletion of protein kinase STK25 ameliorates renal lipotoxicity and protects against diabetic kidney disease. JCI Insight 5:N/A (2020). PubMed: 33170807
- Takiyama Y et al. Impacts of Diabetes and an SGLT2 Inhibitor on the Glomerular Number and Volume in db/db Mice, as Estimated by Synchrotron Radiation Micro-CT at SPring-8. EBioMedicine 36:329-346 (2018). PubMed: 30322799
- Messaoudi S et al. Endothelial Gata5 transcription factor regulates blood pressure. Nat Commun 6:8835 (2015). Functional Studies ; Mouse . PubMed: 26617239