Key features and details
- Expression system: BTI-TN-5B1-4 cells
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
产品名称重组S. cerevisiae KEX2 protease蛋白(Active)
参阅全部 KEX2 protease 蛋白酶
ab96554 from High-5 insect cells contains the same specific activity and recognition sequence specificity as yeast derived KEX2.
1 milligram of recombinant KEX2 contains activity equivalent to at least 40 units of yeast derived KEX2.
Cleaves at the carboxyl side of K/R-R.
纯度> 95 % SDS-PAGE.
>= 95% HPLC analyses.
内毒素水平< 1.000 Eu/µg
序列LPVPAPPMDS SLLPVKEAED KLSINDPLFE RQWHLVNPSF PGSDINVLDL WYNNITGAGV VAAIVDDGLD YENEDLKDNF CAEGSWDFND NTNLPKPRLS DDYHGTRCAG EIAAKKGNNF CGVGVGYNAK ISGIRILSGD ITTEDEAASL IYGLDVNDIY SCSWGPADDG RHLQGPSDLV KKALVKGVTE GRDSKGAIYV FASGNGGTRG DNCNYDGYTN SIYSITIGAI DHKDLHPPYS EGCSAVMAVT YSSGSGEYIH SSDINGRCSN SHGGTSAAAP LAAGVYTLLL EANPNLTWRD VQYLSILSAV GLEKNADGDW RDSAMGKKYS HRYGFGKIDA HKLIEMSKTW ENVNAQTWFY LPTLYVSQST NSTEETLESV ITISEKSLQD ANFKRIEHVT VTVDIDTEIR GTTTVDLISP AGIISNLGVV RPRDVSSEGF KDWTFMSVAH WGENGVGDWK IKVKTTENGH RIDFHSWRLK LFGESIDSSK TETFVFGNDK EEVEPAATES TVSQYSASST SISISATSTS SISIGVETSA IPQTTTASTD PDSDPNTP
氨基酸110 to 667
描述重组S. cerevisiae KEX2 protease蛋白(Active)
Our Abpromise guarantee covers the use of ab96554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Concentration information loading...
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: 0.0555% Calcium chloride, 0.082% Sodium acetate
This product is an active protein and may elicit a biological response in vivo, handle with caution.
复溶The lyophilized protein is stable at room temperature for up to 1 month. Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C.
- KEX 2
- Kexin KEX2
相关性Kex2 in the yeast Saccharomyces cerevisiae is a transmembrane, Ca2+-dependent serine protease of the subtilisin-like pro-protein convertase (SPC) family with specificity for cleavage after paired basic amino acids. At steady state, Kex2 is predominantly localized in late Golgi compartments and initiates the proteolytic maturation of pro-protein precursors that transit the distal secretory pathway. However, Kex2 localization is not static, and its itinerary apparently involves transiting out of the late Golgi and cycling back from post-Golgi endosomal compartments during its lifetime.
细胞定位Golgi apparatus; trans-Golgi network; trans-Golgi network membrane; single-pass type I membrane protein.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.