重组大鼠IL-17A蛋白(Active) (ab282393)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: <= 0.005 Eu/µg
- Active: Yes
- Suitable for: HPLC, MS, SDS-PAGE, Functional Studies, Sandwich ELISA, Cell Culture
描述
-
产品名称
重组大鼠IL-17A蛋白(Active)
参阅全部 IL-17A 蛋白酶 -
生物活性
Fully biologically active determined by the dose dependent induction of IL-6 secretion in NIH-3T3 cells.
ED50 for this effect is ≤ 75.83 ng/ml, corresponding to specific activity of 1.32 X 104 units/mg.
-
纯度
>= 95 % SDS-PAGE.
=95% by HPLC -
内毒素水平
<=0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
-
种属
Rat -
序列
AVLIPQSSVCPNAEANNFLQNVKVNLKVINSLSSKASSRRPSDYLNRSTS PWTLSRNEDPDRYPSVIWEAQCRHQRCVNAEGKLDHHMNSVLIQQEILVL KREPEKCPFTFRVEKMLVGVGCTCVSSIVRHAS -
预测分子量
15 kDa -
氨基酸
18 to 150 -
额外的序列信息
N-terminal glycine
-
-
描述
重组大鼠IL-17A蛋白
技术指标
Our Abpromise guarantee covers the use of ab282393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
HPLC
Mass Spectrometry
SDS-PAGE
Functional Studies
Sandwich ELISA
Cell Culture
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 7.4
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.428% Monobasic dihydrogen potassium phosphate, 10.26% TrehaloseThis product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Store lyophilized form at room temperature. Reconstitute in PBS, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
常规信息
-
别名
- CTLA 8
- CTLA-8
- CTLA8
see all -
功能
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
组织特异性
Restricted to activated memory T-cells. -
序列相似性
Belongs to the IL-17 family. -
翻译后修饰
Found both in glycosylated and nonglycosylated forms. -
细胞定位
Secreted. - Information by UniProt
图片
-
Functional analysis of ab282393.
Fully biologically active determined by the dose dependent induction of IL-6 secretion in NIH-3T3 cells.
ED50 for this effect is ≤ 75.83 ng/ml, corresponding to specific activity of 1.32 X 104 units/mg.
Cell based assay testing is performed on the first lot of protein only and is provided as a reference for protein activity; subsequent lots of protein must pass all biophysical quality control parameters that meet the same parameters as the first lot.
Lot: GR3393274-4
-
SDS-page analysis of ab282393.
-
Sandwich ELISA - Recombinant rat IL-17A protein standard curve.
Background subtracted standard curve using Rat IL-17A Antibody Pair - BSA and Azide free (ab242039) and Recombinant rat IL-17A protein (Active) (ab282393) in sandwich ELISA. The ELISA was performed using the components of the corresponding SimpleStep® kit, which uses the same antibody pair with a different formulation and format.
-
Mass determination by ESI-TOF.
Predicted MW is 15062.23 Da (+/- 10 Da by ESI-TOF). Observed MW is 15063.42.
-
HPLC analysis of ab282393.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab282393 尚未被引用在任何文献中。