Review text: Used for detection of primary antibody in WB after IP with the same antibody. In this case, IP was with goat anti HP1-alpha on Protein G beads, and detection was with goat anti HP1-alpha, and Protein G HRP. Detection was very specific, and eliminated heavy and light chains of denatured antibodies.
Sample: Human Cell lysate - nuclear
Primary antibody (in addition to 'Protein G protein (HRP)')
Primary antibody: Abcam primary antibody: Anti-HP1 alpha antibody - ChIP Grade (ab77256)
Name: Abcam antibody:Protein G protein (HRP)
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提交于 Feb 24 2011