Key features and details
- Expression system: Native
- Purity: > 95% SDS-PAGE
- Suitable for: Conjugation
参阅全部 Protein G 蛋白酶
纯度> 95 % SDS-PAGE.
Chromatographically pure Protein G
蛋白长度Full length protein
Our Abpromise guarantee covers the use of ab7458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
补充说明Molecular Weight: 33,000 daltons Approximately 5 mg of human IgG can be bound by 1 mg of Protein G. Optimal binding of Protein G to antibodies occurs at pH 5-6, with elution at pH 2.5-3. This product was expressed recombinantly in E. coli (non-native).
Concentration information loading...
Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.
复溶Reconstitute with 1ml of distilled water to a concentration of 1mg/ml
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
相关性Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology.
细胞定位Cell Wall and Secreted
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.