重组蛋白G (ab155724)
Key features and details
- Expression system: Escherichia coli
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
描述
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产品名称
重组蛋白G
参阅全部 Protein G 蛋白酶 -
纯度
> 98 % SDS-PAGE.
Lyophilized from 650 µL bulk protein in a 0.2 µm filtered solution of 0.1 M NaH2PO4, pH8.5 with 10% trehalose as protectant. -
内毒素水平
< 1.000 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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预测分子量
31 kDa -
标签
His tag N-Terminus -
额外的序列信息
Contains only one single cysteine at the C-terminus, which is designed for single point attachment coupling for immobilization.
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab155724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.5
Constituents: 1.1998% Sodium Phosphate Monobasic, 10% D-(+)-Trehalose dihydrate -
复溶Reconstitute with sterile deionized water to a final concentration of 20mg/ml.
常规信息
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别名
- IgG binding protein G
- IgG-binding protein G
- Immunoglobulin G binding protein G [Precursor]
see all -
相关性
Protein G is a bacterial protein derived from the cell wall of certain strains of b-hemolytic Streptococcci. It binds with high affinity to the Fc portion of various classes and subclasses of immunoglobulins from a variety of species. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. Protein G binds preferentially to the Fc portion of IgG, but can also bind to the Fab region, making it useful for purification of F(ab') fragments of IgG. Due to it's affinity for the Fc region of many mammalian immunoglobulins, protein G is considered a universal reagent in biochemistry and immunology. -
细胞定位
Cell Wall and Secreted
图片
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SDS-PAGE analysis of reduced ab155724 stained overnight with Coomassie Blue.
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Immobilized Recombinant Protein G, His Tag (ab155724) at 2 μg/mL (100 μL/well) can bind Biotinylated Human IgG1 Fc, Avitag (ab246197) with a linear range of 0.01-0.313 μg/mL
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab155724 被引用在 1 文献中.
- Yasunaga AB et al. Quantitative interpretation of cell rolling velocity distribution. Biophys J 120:2511-2520 (2021). PubMed: 33932434