重组小鼠PKM2蛋白(Active) (ab223006)
Key features and details
- Expression system: Escherichia coli
- Purity: > 85% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, MS
描述
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产品名称
重组小鼠PKM2蛋白(Active)
参阅全部 PKM2 蛋白酶 -
生物活性
Specific activity: > 50,000 pmol/min/ug. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37°C.
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纯度
> 85 % SDS-PAGE.
ab223006 was urified by using conventional chromatography techniques. -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MGSSHHHHHHSSGLVPRGSHMGSMPKPHSEAGTAFIQTQQLHAAMADTFL EHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFS HGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGS GTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVD DGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQ DLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGV RRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICA TQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRM QHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGA IIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDA VLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRV VPVP -
预测分子量
60 kDa including tags -
氨基酸
1 to 531 -
标签
His tag N-Terminus
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相关产品
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Related Products
技术指标
Our Abpromise guarantee covers the use of ab223006 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
SDS-PAGE
Mass Spectrometry
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质谱法
MALDI-TOF -
形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 8.50
Constituents: 0.32% Tris HCl, 1.16% Sodium chloride, 0.02% DTT, 30% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- CTHBP
- Cytosolic thyroid hormone binding protein
- Cytosolic thyroid hormone-binding protein
see all -
功能
Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. -
组织特异性
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. -
通路
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
序列相似性
Belongs to the pyruvate kinase family. -
翻译后修饰
Phosphorylated upon DNA damage, probably by ATM or ATR.
ISGylated. -
细胞定位
Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab223006 尚未被引用在任何文献中。