重组小鼠MIF蛋白(Active) (ab219128)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.050 Eu/µg
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组小鼠MIF蛋白(Active)
参阅全部 MIF 蛋白酶 -
生物活性
The activity is determined by its ability to activate MAP Kinase signaling (via ERK) and is detectable starting at 10 ng/ml. This corresponds to an expected specific activity of 1 x 105 units/mg.
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纯度
> 95 % SDS-PAGE.
Purity determined by reducing and non-reducing SDS-PAGE. -
内毒素水平
< 0.050 Eu/µg -
表达系统
Escherichia coli -
Accession
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蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
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种属
Mouse -
序列
MPMFIVNTNVPRASVPEGFLSELTQQLAQATGKPAQYIAVHVVPDQLMTF SGTNDPCALCSLHSIGKIGGAQNRNYSKLLCGLLSDRLHISPDRVYINYY DMNAANVGWNGSTFA -
预测分子量
13 kDa -
氨基酸
1 to 115 -
额外的序列信息
Non-glycosylated.
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技术指标
Our Abpromise guarantee covers the use of ab219128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Lyophilized -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle.
Constituent: 0.16% Sodium phosphate
Lyophilised from a sterile filtered solution.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Centrifuge vial before opening. Reconstitute with sterile water at 0.1 mg/ml. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. The product works best when used fresh after reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
常规信息
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别名
- GIF
- GLIF
- Glycosylation inhibiting factor
see all -
功能
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
疾病相关
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
序列相似性
Belongs to the MIF family. -
细胞定位
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (0)
ab219128 尚未被引用在任何文献中。