重组小鼠IL-23蛋白(Active) (ab259423)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level: < 0.005 Eu/µg
- Active: Yes
- Suitable for: Functional Studies, HPLC, MS, SDS-PAGE, Cell Culture
描述
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产品名称
重组小鼠IL-23蛋白(Active)
参阅全部 IL-23 蛋白酶 -
生物活性
Fully biologically active as determined by the dose-dependant secretion of IFN-γ by NK-92 cells.
The ED50 is 0.66 ng/mL corresponding to a specific activity of 1.52 x 106 IU/mg.
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纯度
>= 95 % SDS-PAGE.
Purity by HPLC >=95%. -
内毒素水平
< 0.005 Eu/µg -
表达系统
HEK 293 cells -
Accession
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蛋白长度
Full length protein -
无动物成分
Yes -
无载体
是 -
性质
Recombinant -
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氨基酸序列 1
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种属
Mouse -
预测分子量
36 kDa -
分子量信息
M + 2.62 Da (Calc. mass 35848.48 Da). MWELEKDVYVVEVDWTPDAPGETVNLTCDTPEEDDITWTSDQRHGVIGSGKTLTITVKEFLDAGQYTCHKGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVQRNMDLKFNIKSSSSSPDSRAVTCGMASLSAEKVTLDQRDYEKYSVSCQEDVTCPTAEETLPIELALEARQQNKYENYSTSFFIRDIIKPDPPKNLQMKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKMKETEEGCNQKGAFLVEKTSTEVQCKGGNVCVQAQDRYYNSSCSKWACVPCRVRS -
氨基酸
23 to 335 -
额外的序列信息
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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氨基酸序列 2
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种属
Mouse -
预测分子量
20 kDa -
分子量信息
M + 0.61 Da (Calc. mass 19716.39 Da). -
氨基酸
22 to 196 -
额外的序列信息
N-terminal glycine. Full-length mature chain lacking the signal peptide.
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技术指标
Our Abpromise guarantee covers the use of ab259423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
Functional Studies
HPLC
Mass Spectrometry
SDS-PAGE
Cell Culture
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形式
Lyophilized -
补充说明
This protein is filter sterilised prior to aliquoting and lyophilisation. All aliquoting and lyophilisation steps are performed in a sterile environment.
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Concentration information loading...
制备和贮存
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稳定性和存储
Shipped at Room Temperature. Store at Room Temperature.
pH: 6.00
Constituents: 0.727% Dibasic monohydrogen potassium phosphate, 0.248% Monobasic dihydrogen potassium phosphate, 10.26% Trehalose
Buffer lyophilized from.This product is an active protein and may elicit a biological response in vivo, handle with caution.
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复溶Reconstitute with phosphate buffered saline.Store lyophilized form at room temperature. Reconstitute, aliquot and store at -80°C for 12 months or +4°C for 1 week.Avoid repeated freeze-thaw. Lyophilized contents may appear as either a translucent film or a white powder. This variance does not affect the quality of the product.
常规信息
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别名
- IL 23
- IL 23 A
- IL 23 subunit alpha
see all -
功能
Associates with IL12B to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis. -
组织特异性
Secreted by activated dendritic and phagocytic cells and keratinocytes. Also expressed by dermal Langerhans cells (at protein level). -
序列相似性
Belongs to the IL-6 superfamily. -
发展阶段
Expressed by newborns dendritic cells. -
细胞定位
Secreted. Secreted upon association with IL12B. - Information by UniProt
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab259423 尚未被引用在任何文献中。