Key features and details
- Expression system: Baculovirus
- Purity: > 65% SDS-PAGE
- Active: Yes
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE, WB
参阅全部 XIAP 蛋白酶
Specific Activity: 0.0015 pmol/min/µg Assay conditions: 4 mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50mM Tris pH 7.4. Specific Activity: 0.0015 pmol/min/µg Assay conditions: 4 mM ATP, 5mM MgCl2 (both required for reaction), 1mM DTT, E1 (0.5 µg), E2 (2.8 µg), Biotinylated Ubiquitin (1 µg) in 50mM Tris pH 7.4.
纯度> 65 % SDS-PAGE.
蛋白长度Full length protein
预测分子量57 kDa including tags
氨基酸2 to 497
标签DDDDK tag N-Terminus
Our Abpromise guarantee covers the use of ab95933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Shipped on Dry Ice. Store at -80°C.
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.58% Sodium chloride
104 µg/ml FLAG peptide
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- AP 13
- Apoptosis Inhibitor 3
功能Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.
组织特异性Ubiquitous, except peripheral blood leukocytes.
疾病相关Defects in XIAP are the cause of lymphoproliferative syndrome X-linked type 2 (XLP2) [MIM:300635]. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.
序列相似性Belongs to the IAP family.
Contains 3 BIR repeats.
Contains 1 RING-type zinc finger.
结构域The first BIR domain is involved in interaction with TAB1/MAP3K7IP1 and is important for dimerization. The second BIR domain is sufficient to inhibit caspase-3 and caspase-7, while the third BIR is involved in caspase-9 inhibition. The interactions with SMAC and PRSS25 are mediated by the second and third BIR domains.
翻译后修饰Ubiquitinated and degraded by the proteasome in apoptotic cells.
Phosphorylation by PKB/AKT protects XIAP against ubiquitination and protects the protein against proteasomal degradation.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.