Application
Western blot
Application: Western blot
Gel Running Conditions: Reduced Denaturing (12% gel)
Blocking step: 5% skim milk as blocking agent for 1 hour at RT
Primary antibody:
Abcam antibody ab4808 Anti-VP16 tag antibody
Dilution: 1/1000 Diluent: TBS + 0.05% sodium azide Incubation: 1 hour at RT
Secondary antibody:
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed, abcam antibody Cat#: ab7091
Dilution: 1/2000 in TBS with 0.05% sodium azide Incubation: 1 hour at RT
Detection
Detection method: BCIP/NBT Exposure: 2 minutes
Bands
Specific: 36kDa
Image: MW Ladder: SuperMarker2700 (5ul)
lane #1: 100 ng of ab81832 lane #2: 50 ng of ab81832 lane #3: 25 ng of ab81832
Gel Running Conditions: Reduced Denaturing (12% gel)
Blocking step: 5% skim milk as blocking agent for 1 hour at RT
Primary antibody:
Abcam antibody ab4808 Anti-VP16 tag antibody
Dilution: 1/1000 Diluent: TBS + 0.05% sodium azide Incubation: 1 hour at RT
Secondary antibody:
Goat Anti-Rabbit IgG H&L (Alkaline Phosphatase) preadsorbed, abcam antibody Cat#: ab7091
Dilution: 1/2000 in TBS with 0.05% sodium azide Incubation: 1 hour at RT
Detection
Detection method: BCIP/NBT Exposure: 2 minutes
Bands
Specific: 36kDa
Image: MW Ladder: SuperMarker2700 (5ul)
lane #1: 100 ng of ab81832 lane #2: 50 ng of ab81832 lane #3: 25 ng of ab81832
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 24 2021