重组人VEGF Receptor 3蛋白(ab95247)
Key features and details
- Expression system: Baculovirus infected insect cells
- Purity: > 70% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
描述
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产品名称
重组人VEGF Receptor 3蛋白
参阅全部 VEGF Receptor 3 蛋白酶 -
生物活性
Specific Activity: 17 pmol/min/µg. Assay conditions: VEGFR3 was incubated with a substrate (Tyr peptide 4 for 1h at RT in 1Xkinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
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纯度
> 70 % SDS-PAGE.
Affinity purified. -
表达系统
Baculovirus infected insect cells -
蛋白长度
Protein fragment -
无动物成分
No -
性质
Recombinant -
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种属
Human -
氨基酸
798 to 1298 -
标签
GST tag N-Terminus
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技术指标
Our Abpromise guarantee covers the use of ab95247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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应用
SDS-PAGE
Functional Studies
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形式
Liquid -
Concentration information loading...
制备和贮存
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稳定性和存储
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.0462% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.395% Tris HCl, 0.05% Tween, 20% Glycerol (glycerin, glycerine), 0.8004% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
常规信息
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别名
- EC 2.7.10.1
- flt 4
- FLT-4
see all -
功能
Receptor for VEGFC. Has a tyrosine-protein kinase activity. -
组织特异性
Placenta, lung, heart, and kidney, does not seem to be expressed in pancreas and brain. -
疾病相关
Defects in FLT4 are the cause of lymphedema hereditary type 1A (LMPH1A) [MIM:153100]; also known as Nonne-Milroy lymphedema or Milroy disease. Hereditary lymphedema is a chronic disabling condition which results in swelling of the extremities due to altered lymphatic flow. Patients with lymphedema suffer from recurrent local infections and physical impairment.
Note=Defects in FLT4 are found in juvenile hemangioma. Juvenile hemangiomas are the most common tumors of infancy, occurring as many as 10% of all births. These benign vascular lesions enlarge rapidly during the first year of life by hyperplasia of endothelial cells and attendant pericytes, and then spontaneously involute over a period of years, leaving loose fibrofatty tissue. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
细胞定位
Membrane. - Information by UniProt
图片
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The specific activity of ab95247 was found to be 17 pmol/min/µg by carrying out functional studies. The assay conditions were; VEGFR3 was incubated with a substrate (Tyr peptide 4) for 1h at RT in 1Xkinase buffer supplemented with ATP. Developer solution was added to reaction and reaction was stopped after 1h of incubation at RT.
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ab95247 was analysed by SDS-PAGE. Lane 1; ab95247 at 4µg. Lane 2; protein molecular weight markers. ab95247 has a predicted molecular weight of 82 kDa and is >70% pure as assessed by SDS-PAGE.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab95247 尚未被引用在任何文献中。