重组人TGF beta 1蛋白(Active) (ab50038)
Key features and details
- Expression system: CHO cells
- Purity: > 98% SDS-PAGE
- Endotoxin level: < 1.000 Eu/µg
- Active: Yes
- Suitable for: HPLC, SDS-PAGE, Functional Studies
描述
-
产品名称
重组人TGF beta 1蛋白(Active)
参阅全部 TGF beta 1 蛋白酶 -
生物活性
The ED50, as determined by TGF beta 1’s ability to inhibit the mouse IL-4-dependent proliferation of mouse HT-2 cells, is ≤ 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg
-
纯度
> 98 % SDS-PAGE.
>98% HPLC analyses. -
内毒素水平
< 1.000 Eu/µg -
表达系统
CHO cells -
Accession
-
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CSSSALDTNY CFSSTEKNCC VRQLYIDFRK DLGWKWIHEP KGYHANFCLG PCPYIWSLDT QYSKVLALYN QHNPGASAAP CCVPQALEPL PIVYYVGRKP KVEQLSNMIV RSCKCS -
预测分子量
25 kDa -
氨基酸
279 to 390 -
额外的序列信息
ab50038 is composed of two identical 112 amino acid polypeptide chains linked by a single disulfide bond. It is the mature protein after cleavage of the signal peptide and latency-associated peptide.
-
相关产品
-
Related Products
技术指标
Our Abpromise guarantee covers the use of ab50038 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
HPLC
SDS-PAGE
Functional Studies
-
形式
Lyophilized -
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Working aliquots stored with a carrier protein are stable for at least 3 months at -20°C to -80°C..
This product is an active protein and may elicit a biological response in vivo, handle with caution.
-
复溶Centrifuge the vial prior to opening. For lot GR18648 Reconstitute in 10mM Citric Acid, pH 3.0 to 0.1-1.0 mg/ml. For all other lots reconstitute in water to a concentration of 0.1- 1.0 mg/ml. Do not vortex. If you have any questions regaring the reconstitution of ab50038 please contact scientific support.
常规信息
-
别名
- Cartilage-inducing factor
- CED
- Differentiation inhibiting factor
see all -
功能
Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. -
组织特异性
Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. -
疾病相关
Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. -
序列相似性
Belongs to the TGF-beta family. -
翻译后修饰
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. -
细胞定位
Secreted > extracellular space > extracellular matrix. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
SDS download
-
Datasheet download
文献 (1)
ab50038 被引用在 1 文献中.
- Yang XL et al. Optimal pressure in penile rehabilitation with a vacuum erection device: evidence based on a rat model. Asian J Androl N/A:N/A (2019). PubMed: 30924453