重组人TFII I蛋白(ab152444)
Key features and details
- Expression system: Wheat germ
- Suitable for: ELISA, SDS-PAGE, WB
描述
-
产品名称
重组人TFII I蛋白 -
表达系统
Wheat germ -
蛋白长度
Full length protein -
无动物成分
No -
性质
Recombinant -
-
种属
Human -
序列
MAQVAMSTLPVEDEESSESRMVVTFLMSALESMCKELAKSKAEVACIAVY ETDVFVVGTERGRAFVNTRKDFQKDFVKYCVEEEEKAAEMHKMKSTTQAN RMSVDAVEIETLRKTVEDYFCFCYGKALGKSTVVPVPYEKMLRDQSAVVV QGLPEGVAFKHPENYDLATLKWILENKAGISFIIKRPFLEPKKHVGGRVM VTDADRSILSPGGSCGPIKVKTEPTEDSGISLEMAAVTVKEESEDPDYYQ YNIQGPSETDDVDEKQPLSKPLQGSHHSSEGNEGTEMEVPAEDDDYSPPS KRPKANELPQPPVPEPANAGKRKVREFNFEKWNARITDLRKQVEELFERK YAQAIKAKGPVTIPYPLFQSHVEDLYVEGLPEGIPFRRPSTYGIPRLERI LLAKERIRFVIKKHELLNSTREDLQLDKPASGVKEEWYARITKLRKMVDQ LFCKKFAEALGSTEAKAVPYQKFEAHPNDLYVEGLPENIPFRSPSWYGIP RLEKIIQVGNRIKFVIKRPELLTHSTTEVTQPRTNTPVKEDWNVRITKLR KQVEEIFNLKFAQALGLTEAVKVPYPVFESNPEFLYVEGLPEGIPFRSPT WFGIPRLERIVRGSNKIKFVVKKPELVISYLPPGMASKINTKALQSPKRP RSPGSNSKVPEIEVTVEGPNNNNPQTSAVRTPTQTNGSNVPFKPRGREFS FEAWNAKITDLKQKVENLFNEKCGEALGLKQAVKVPFALFESFPEDFYVE GLPEGVPFRRPSTFGIPRLEKILRNKAKIKFIIKKPEMFETAIKESTSSK SPPRKINSSPNVNTTASGVEDLNIIQVTIPDDDNERLSKVEKARQLREQV NDLFSRKFGEAIGMGFPVKVPYRKITINRGCVVVDGMPPGVSFKAPSYLE ISSMRRILDSAEFIKFTVIRPFPGLVVNNQLVDQSESEGPVIQESAEPSQ LEVPATEEIKETDGSSQIKQEPDPTW -
预测分子量
137 kDa including tags -
氨基酸
1 to 976
-
技术指标
Our Abpromise guarantee covers the use of ab152444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
应用
ELISA
SDS-PAGE
Western blot
-
形式
Liquid -
补充说明
-
Concentration information loading...
制备和贮存
-
稳定性和存储
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 8.00
Constituents: 0.31% Glutathione, 0.79% Tris HCl
常规信息
-
别名
- BAP 135
- BAP-135
- BAP135
see all -
功能
Interacts with the basal transcription machinery by coordinating the formation of a multiprotein complex at the C-FOS promoter, and linking specific signal responsive activator complexes. Promotes the formation of stable high-order complexes of SRF and PHOX1 and interacts cooperatively with PHOX1 to promote serum-inducible transcription of a reporter gene deriven by the C-FOS serum response element (SRE). Acts as a coregulator for USF1 by binding independently two promoter elements, a pyrimidine-rich initiator (Inr) and an upstream E-box. Required for the formation of functional ARID3A DNA-binding complexes and for activation of immunoglobulin heavy-chain transcription upon B-lymphocyte activation. -
组织特异性
Ubiquitous. Isoform 1 is strongly expressed in fetal brain, weakly in adult brain, muscle, and lymphoblasts and is almost undetectable in other adult tissues, while the other isoforms are equally expressed in all adult tissues. -
疾病相关
Note=GTF2I is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of GTF2I may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease. -
序列相似性
Belongs to the TFII-I family.
Contains 6 GTF2I-like repeats. -
翻译后修饰
Transiently phosphorylated on tyrosine residues by BTK in response to B-cell receptor stimulation. Phosphorylation on Tyr-248 and Tyr-398, and perhaps, on Tyr-503 contributes to BTK-mediated transcriptional activation.
Sumoylated. -
细胞定位
Cytoplasm. Nucleus. Colocalizes with BTK in the cytoplasm. - Information by UniProt
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
-
Datasheet download
文献 (0)
ab152444 尚未被引用在任何文献中。