Key features and details
- Expression system: Escherichia coli
- Active: Yes
- Suitable for: WB, Functional Studies
参阅全部 Sumo 3 蛋白酶
生物活性The final fraction of enzyme contains a single polypeptide band of 11 kDa.
蛋白长度Full length protein
Our Abpromise guarantee covers the use of ab3802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
补充说明The final fraction of enzyme contains a single polypeptide band of 11 kDa.
Concentration information loading...
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.68% Imidazole
Constituents: 0.0087% PMSF, 0.0154% DTT, 0.158% Tris HCl, 10% Glycerol, 0.58% Sodium chloride
This product is an active protein and may elicit a biological response in vivo, handle with caution.
- Small ubiquitin like modifier 3
- Small ubiquitin related modifier 3
- Small ubiquitin-related modifier 3
功能Ubiquitin-like protein which can be covalently attached to target lysines either as a monomer or as a lysine-linked polymer. Does not seem to be involved in protein degradation and may function as an antagonist of ubiquitin in the degradation process. Plays a role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by an E3 ligase such as PIAS1-4, RANBP2 or CBX4.
组织特异性Expressed predominantly in liver.
序列相似性Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain.
翻译后修饰Polymeric chains can be formed through Lys-11 cross-linking.
Cleavage of precursor form by SENP1, SENP2 or SENP5 is necessary for function.
- Information by UniProt
Left Lane: Topo I protein (Topo I fragment: ab3828)
Note: Topo I is S35-Met labeled.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.