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Immunology Cell Type Markers CD Adhesion

重组人Semaphorin 4D/CD100蛋白(ab97413)

  • Datasheet
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Key features and details

  • Expression system: CHO cells
  • Purity: > 95% SDS-PAGE
  • Endotoxin level: < 0.100 Eu/µg
  • Suitable for: SDS-PAGE, HPLC

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描述

  • 产品名称

    重组人Semaphorin 4D/CD100蛋白
    参阅全部 Semaphorin 4D/CD100 蛋白酶
  • 纯度

    > 95 % SDS-PAGE.
    Purity: Greater than 97% by SDS-PAGE gel and HPLC analyses. SDS-PAGE analysis run under non-reducing conditions shows a mixture of disulfide linked dimer and monomer.
  • 内毒素水平

    < 0.100 Eu/µg
  • 表达系统

    CHO cells
  • Accession

    Q92854
  • 蛋白长度

    Protein fragment
  • 无动物成分

    No
  • 性质

    Recombinant
    • 种属

      Human
    • 序列

      FAPIPRITWE HREVHLVQFH EPDIYNYSAL LLSEDKDTLY IGAREAVFAV NALNISEKQH EVYWKVSEDK KAKCAEKGKS KQTECLNYIR VLQPLSATSL YVCGTNAFQP ACDHLNLTSF KFLGKNEDGK GRCPFDPAHS YTSVMVDGEL YSGTSYNFLG SEPIISRNSS HSPLRTEYAI PWLNEPSFVF ADVIRKSPDS PDGEDDRVYF FFTEVSVEYE FVFRVLIPRI ARVCKGDQGG LRTLQKKWTS FLKARLICSR PDSGLVFNVL RDVFVLRSPG LKVPVFYALF TPQLNNVGLS AVCAYNLSTA EEVFSHGKYM QSTTVEQSHT KWVRYNGPVP KPRPGACIDS EARAANYTSS LNLPDKTLQF VKDHPLMDDS VTPIDNRPRL IKKDVNYTQI VVDRTQALDG TVYDVMFVST DRGALHKAIS LEHAVHIIEE TQLFQDFEPV QTLLLSSKKG NRFVYAGSNS GVVQAPLAFC GKHGTCEDCV LARDPYCAWS PPTATCVALH QTESPSRGLI QEMSGDASVC PDKSKGSYRQ HFFKHGGTAE LKCSQKSNLA RVFWKFQNGV LKAESPKYGL MGRKNLLIFN LSEGDSGVYQ CLSEERVKNK TVFQVVAKHV LEVKVVPKPV VAPTLSVVQT EGSRIATKVL VASTQGSSPP TPAVQATSSG AITLPPKPAP TGTSCEPKIV INTVPQLHSE KTMYLKSSDN R
    • 预测分子量

      79 kDa
    • 氨基酸

      24 to 734

技术指标

Our Abpromise guarantee covers the use of ab97413 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 应用

    SDS-PAGE

    HPLC

  • 形式

    Lyophilized
  • 补充说明

    This product was previously labelled as Semaphorin 4D.

    For lot specific reconstitution information please contact our Scientific Support Team.

  • Concentration information loading...

制备和贮存

  • 稳定性和存储

    Shipped at 4°C. The lyophilized protein is stable for a few weeks at room temperature. Store at -20°C or -80°C. Avoid freeze / thaw cycle. Please see notes section.

    Constituent: 0.164% Sodium phosphate

  • 复溶
    For lot specific reconstitution information please contact our Scientific Support Team.

常规信息

  • 别名

    • A8
    • BB 18
    • BB18
    • CD 100
    • CD100
    • CD100 antigen
    • Coll 4
    • COLL4
    • Collapsin 4
    • Collapsin4
    • GR3
    • Leukocyte activation antigen CD100
    • M sema G
    • MSEMA
    • SEM4D_HUMAN
    • Sema 4d
    • Sema domain immunoglobulin domain Ig transmembrane domain TM and short cytoplasmic domain semaphorin 4D
    • Sema H
    • SEMA J
    • Sema4d
    • Semacl 2
    • Semacl2
    • SemaH
    • SEMAJ
    • Semaphorin 4D
    • Semaphorin C like 2
    • Semaphorin H
    • Semaphorin J
    • Semaphorin-4D
    • Semaphorin4D
    • SemaphorinJ
    • Semcl 2
    • Semcl2
    see all
  • 功能

    May play a functional role in the immune system, as well as in the nervous system. Induces B-cells to aggregate and improves their viability in vitro.
  • 组织特异性

    Strongly expressed in skeletal muscle, peripheral blood lymphocytes, spleen, and thymus and also expressed at lower levels in testes, brain, kidney, small intestine, prostate, heart, placenta, lung and pancreas, but not in colon and liver.
  • 序列相似性

    Belongs to the semaphorin family.
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 PSI domain.
    Contains 1 Sema domain.
  • 细胞定位

    Membrane.
  • Target information above from: UniProt accession Q92854 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

  • Datasheet download

    Download

文献 (0)

发表研究结果有使用 ab97413?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab97413 尚未被引用在任何文献中。

客户评价及客户问答

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Question

  Hope this e-mail finds you well!   Kindly look into the below enquiry from one of our customer & advise us on it, enabling us to update her accordingly.   Looking forward towards your valued response on it.   With Best Regards,   My enquiry is about SEMAPHOHRIN 4D , protein, detection in Gingival crevicular fluid (GCF). GCF is present in gum/gingival tissues and is collected in microlitres and can be diluted in saline or so.   Research/pilot study regarding semaphorin 4D presence in GCF is to be done to confirm its presence in GCF so that I can buy the SEMAPHORIN 4D antibody product to further carry out my research.I want to use semaphorin 4D as a biomarker in GCF during inflammation.   Kindly send me the information as soon as possible.   Regards  

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Abcam community

Verified customer

Asked on Dec 14 2011

Answer

Thank you and your customer for your inquiry. I am happy to confirm that we have 8 antibody for SEMAPHORIN 4D in our catalogue. Please find a list here: https://www.abcam.com/index.html?pageconfig=searchresults&search=SEMAPHORIN 4D&pt=1&sk=targ&sv=Semaphorin 4D&sn=Semaphorin 4D&l=0&fViewMore=1 These antibodies are all tested for human samples and a variety of applications. If your customer would like further help in choosing the right antibody for their experiment, I will be happy to help. In this case please ask your customer to provide more information concerning species and application of the planned experiments. We also offer a SEMAPHORIN 4D protein (ab97413, Recombinant fragment corresponding to amino acids 24-734 (the extracellular soluble domain) of Human Semaphorin 4D) that might be useful as positive control: Click here (or use the following: https://www.abcam.com/Recombinant-Human-Semaphorin-4DCD100-protein-ab97413.html). I hope this information was helpful. Please do not hesitate to contact me again with any further questions.

Read More

Abcam Scientific Support

回复于 Dec 14 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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